Shkotova Lyudmyla V, Piechniakova Nataliia Y, Kukla Oleksandr L, Dzyadevych Sergei V
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03143 Kyiv, Ukraine.
Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Str., 03143 Kyiv, Ukraine; National Taras Shevchenko University of Kyiv, Educational and Scientific Centre «Institute of Biology», 2 Glushkova Str., Block 12, 03022 Kyiv, Ukraine.
Food Chem. 2016 Apr 15;197(Pt A):972-8. doi: 10.1016/j.foodchem.2015.11.066. Epub 2015 Nov 14.
An amperometric multi-biosensor based on lactate and glucose oxidases has been developed for determination of lactate and glucose in wine. Gold thin-film amperometric electrodes were used as multi-transducers. Analytical characteristics of the multi-biosensor developed were studied. The minimum detectable concentration was 5×10(-6) mol/l for both glucose and lactate. High reproducibility and storage stability of the multi-biosensor are demonstrated in this paper. Lactate and glucose were determined in wine, and a good correlation was obtained with concentrations determined using high-performance liquid chromatography (correlation coefficient for glucose R(2)=0.998, for lactate R(2)=0.718).
一种基于乳酸氧化酶和葡萄糖氧化酶的安培型多生物传感器已被开发用于测定葡萄酒中的乳酸和葡萄糖。金薄膜安培电极用作多传感器。研究了所开发的多生物传感器的分析特性。葡萄糖和乳酸的最低检测浓度均为5×10(-6) mol/l。本文展示了该多生物传感器具有高重现性和储存稳定性。对葡萄酒中的乳酸和葡萄糖进行了测定，与使用高效液相色谱法测定的浓度具有良好的相关性（葡萄糖的相关系数R(2)=0.998，乳酸的相关系数R(2)=0.718）。
