基于点测试和智能手机图像的低成本原位数字图像法，用于测定巴西亚马逊地区本土和外来水果中的抗坏血酸。
Low cost in situ digital image method, based on spot testing and smartphone images, for determination of ascorbic acid in Brazilian Amazon native and exotic fruits.
机构信息
Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Rua Augusto Corrêa, 01 - Setor Básico, CEP 66075-110 Belém, PA, Brazil.
Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Rua Augusto Corrêa, 01 - Setor Básico, CEP 66075-110 Belém, PA, Brazil.
出版信息
Food Chem. 2019 Jul 1;285:340-346. doi: 10.1016/j.foodchem.2019.01.167. Epub 2019 Feb 1.
In this work, we propose a method that employed a smartphone to capture images obtained from a colorimetric spot test to determine ascorbic acid (AA) in Brazilian Amazon native and exotic fruits. The spot test reaction was based on reduction of Fe(III) by AA and further complexation with 1,10-phenanthroline. After optimization, the limit of detection was 8.5 × 10 mol L. Brazilian Amazon native fruits such as bacuri, cupuaçu, muruci, yellow mombin, as well as others as cashew, mango, orange and passion fruit, were analyzed. In order to determine the accuracy of this method, iodometric titration was used; results were in close agreement with a confidence level of 95% (paired t-test). Moreover, recoveries ranged from 87.1 to 116%. The method is economic, environmentally friendly, and portable, and might useful for small producers and family agriculture businesses unable to afford specialized laboratory analysis in the north region of Brazilian Amazon.
在这项工作中，我们提出了一种方法，该方法使用智能手机拍摄比色斑点测试获得的图像，以确定巴西亚马逊地区本地和外来水果中的抗坏血酸 (AA)。斑点测试反应基于 AA 将 Fe(III)还原，然后与 1,10-菲啰啉进一步络合。经过优化，检测限为 8.5×10-6 mol L。分析了巴西亚马逊地区的本地水果，如bacuri、cupuaçu、muruci、yellow mombin 以及腰果、芒果、橙子和百香果等。为了确定该方法的准确性，使用了碘量滴定法；结果与置信水平为 95%（配对 t 检验）非常吻合。此外，回收率在 87.1%至 116%之间。该方法经济、环保且便携，对于无法承担巴西亚马逊北部地区专业实验室分析费用的小生产者和家庭农业企业可能非常有用。
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