College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, PR China; Laboratory of Quality & Safety Risk Assessment for Agro-products (Yangling), Ministry of Agriculture, Yangling, Shaanxi 712100, PR China.
Food Chem. 2021 May 30;345:128839. doi: 10.1016/j.foodchem.2020.128839. Epub 2020 Dec 11.
A ratiometric fluorescent sensor was facilely fabricated using innate fluorescence of carbendazim (MBC) and fluorescent UiO-67 to sensitively and selectively detect MBC in food matrixes. The innate fluorescence of MBC provided a signal at 311 nm (F), and the fluorescent UiO-67 at 408 nm (F) could recognize MBC through π-π stacking inducing fluorescent quenching relied on photoelectron transfer (PET). The ratio (F/F) of the fluorescence enhancement of MBC and the quenching of UiO-67 linearly responded to the MBC concentrations of 0-47.6 μmol/L with a low limit of detection (LOD) of 3.0 × 10 μmol/L. The reverse response signals of the sensor enhanced the sensitivity toward MBC and presented remarkable anti-interference capability in complex matrices. The as-prepared sensor was applied to detect MBC residues in apple, cucumber and cabbage, obtaining satisfactory accuracy and precision with the recovery of 90.82-103.45% and RSDs of lower than 3.03%.
比率型荧光传感器是使用多菌灵（MBC）的固有荧光和荧光 UiO-67 制备的，用于灵敏和选择性地检测食品基质中的 MBC。MBC 的固有荧光在 311nm 处提供信号（F），而荧光 UiO-67 在 408nm 处（F）可以通过依赖光电转移（PET）的π-π 堆积识别 MBC，从而导致荧光猝灭。MBC 的荧光增强和 UiO-67 的猝灭的比率（F/F）与 MBC 浓度在 0-47.6μmol/L 范围内呈线性响应，检测限（LOD）低至 3.0×10μmol/L。传感器的反向响应信号增强了对 MBC 的灵敏度，并在复杂基质中表现出显著的抗干扰能力。所制备的传感器用于检测苹果、黄瓜和白菜中的 MBC 残留，回收率为 90.82-103.45%，RSD 低于 3.03%，具有令人满意的准确性和精密度。
