Dipartimento di Scienze del Farmaco, Università degli Studi del Piemonte Orientale "A. Avogadro", Largo Donegani 2, 28100 Novara, Italy.
UMR 1083 Sciences Pour l'Œnologie, Polyphenols Platform, INRA, Montpellier SupAgro, Université de Montpellier2, Montpellier, France.
Food Chem. 2019 Jul 1;285:423-430. doi: 10.1016/j.foodchem.2019.01.175. Epub 2019 Feb 6.
The study was designed to evaluate how the distillation process is able to affect the composition and bioactivity of oligosaccharides contained in the grape seeds. Different oligosaccharidic fractions have been extracted both before and after grape pomace distillation in order to valorize this by-product. A multistep solid-phase extraction approach (C-18 and carbograph cartridges) has been applied to purify and fractionate the oligosaccharidic compounds. Chemical characterization of the fractions was performed using a UPLC-ESI-MS method. Complex oligosaccharides consist principally of neutral oligosaccharides rich in arabinose and glucose. Then, the oligosaccharides contained in the different fractions as potential functional ingredients with prebiotic activity toward well-known probiotic bacteria, such as Lactobacillus acidophilus and Lactobacillus plantarum, were evaluated. Data showed how, in some combination, oligosaccharidic fractions obtained may be considered a novel "functional ingredient" with potential prebiotic activity mainly towards L. acidophilus.
本研究旨在评估葡萄籽油在蒸馏过程中对葡萄籽油中寡糖组成和生物活性的影响。为了对该副产物进行增值，我们分别在葡萄渣蒸馏前后提取了不同的寡糖馏分。采用多步固相萃取法（C-18 和 Carbograph 柱）对寡糖化合物进行纯化和分级。采用 UPLC-ESI-MS 法对馏分进行化学表征。结果表明，复杂的寡糖主要由富含阿拉伯糖和葡萄糖的中性寡糖组成。然后，评估了不同馏分中所含的寡糖作为潜在的功能性成分，对包括嗜酸乳杆菌和植物乳杆菌在内的已知益生菌具有益生元活性。结果表明，在某些组合中，获得的寡糖馏分可被视为具有潜在益生元活性的新型“功能性成分”，主要针对嗜酸乳杆菌。
