Dipartimento di Scienze del Farmaco, Università degli Studi del Piemonte Orientale "A. Avogadro", Largo Donegani 2, 28100 Novara, Italy.
Dipartimento di Scienze del Farmaco, Università degli Studi del Piemonte Orientale "A. Avogadro", Largo Donegani 2, 28100 Novara, Italy.
Food Chem. 2021 May 15;344:128588. doi: 10.1016/j.foodchem.2020.128588. Epub 2020 Nov 9.
Grape seeds are among the main constituents of grape pomace, ranging between 20% and 30% of the wet matrix; however, their oligosaccharide composition has not been studied. This paper describes the purification and the identification of low molecular weight oligosaccharides contained in an EtOH/water extract of grape seeds. A sequential two-step purification by size exclusion chromatography was carried out to fractionate compounds according to molecular weights. Chemical characterization of the combined fractions was performed by Magnetic Resonance Spectroscopy and Gas Chromatography-Mass Spectrometry analyses. The separation process gave two fractions abundant in sucrose and glucose. A third fraction containing trisaccharides was acetylated allowing the purification of the main trisaccharide. The structure elucidation of the acetylated product made it possible to identify gentianose, a predominant carbohydrate reserve found in the storage roots of perennial Gentiana lutea. Grape seeds are wine industry by-products and the obtained results suggest the importance of their recovery.
葡萄籽是葡萄废渣的主要成分之一，占湿基质的 20%至 30%；然而，其低聚糖的组成尚未得到研究。本文描述了从葡萄籽的 EtOH/水溶液提取物中纯化和鉴定低分子量寡糖的方法。通过尺寸排阻色谱进行了两步顺序纯化，根据分子量对化合物进行分级。通过核磁共振波谱和气相色谱-质谱分析对合并的馏分进行化学表征。分离过程得到了富含蔗糖和葡萄糖的两种馏分。第三种含有三糖的馏分被乙酰化，从而可以纯化主要的三糖。乙酰化产物的结构阐明使得能够鉴定龙胆三糖，这是多年生龙胆根茎中主要的碳水化合物储备。葡萄籽是葡萄酒工业的副产品，所得结果表明回收它们的重要性。
