Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia (CBiot), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil; Programa de Pós-Graduação em Biologia Celular e Molecular, CBiot, UFRGS, Porto Alegre, RS, Brazil.
Laboratório de Genômica Estrutural e Funcional, Centro de Biotecnologia (CBiot), Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil; Departamento de Biologia Molecular e Celular, Instituto de Biociências, UFRGS, Porto Alegre, RS, Brazil.
J Proteomics. 2018 Mar 20;175:127-135. doi: 10.1016/j.jprot.2017.12.022. Epub 2018 Jan 6.
Mesocestoides corti is a widely used model for the study of cestode biology, and its transition from the larval tetrathyridium (TT) stage to the strobilated, adult worm (ST) stage can be induced and followed in vitro. Here, a proteomic approach was used to describe and compare M. corti TT and ST protein repertories. Overall, 571 proteins were identified, 238 proteins in TT samples and 333 proteins in ST samples. Among the identified proteins, 207 proteins were shared by TTs and STs, while 157 were stage-specific, being 31 exclusive from TTs, and 126 from STs. Functional annotation revealed fundamental metabolic differences between the TT and the ST stages. TTs perform functions related mainly to basic metabolism, responsible for growth and vegetative development by asexual reproduction. STs, in contrast, perform a wider range of functions, including macromolecule biosynthetic processes, gene expression and control pathways, which may be associated to its proglottization/segmentation, sexual differentiation and more complex physiology. Furthermore, the generated results provided an extensive list of cestode proteins of interest for functional studies in M. corti. Many of these proteins are novel candidate diagnostic antigens, and/or potential targets for the development of new and more effective antihelminthic drugs.
Cestodiases are parasitic diseases with serious impact on human and animal health. Efforts to develop more effective strategies for diagnosis, treatment or control of cestodiases are impaired by the still limited knowledge on many aspects of cestode biology, including the complex developmental processes that occur in the life cycles of these parasites. Mesocestoides corti is a good experimental model to study the transition from the larval to the adult stage, called strobilation, which occur in typical cestode life-cycles. The performed proteomics approach provided large-scale identification and quantification of M. corti proteins. Many stage-specific or differentially expressed proteins were detected in the larval tetrathyridium (TT) stage and in the strobilated, adult worm (ST) stage. Functional comparative analyses of the described protein repertoires shed light on function and processes associated to specific features of both stages, such as less differentiation and asexual reproduction in TTs, and proglottization/segmentation and sexual differentiation in ST. Moreover, many of the identified stage-specific proteins are useful as cestode developmental markers, and are potential targets for development of novel diagnostic methods and therapeutic drugs for cestodiases.
Mesocestoides corti 是研究绦虫生物学的常用模型，其可从幼虫四叶期（TT）过渡到有棘绦虫期（ST），这一过程可在体外诱导和跟踪。在这里，我们采用蛋白质组学方法来描述和比较 M. corti TT 和 ST 的蛋白质组成。总体而言，共鉴定了 571 种蛋白质，TT 样本中有 238 种，ST 样本中有 333 种。在鉴定的蛋白质中，有 207 种蛋白质在 TT 和 ST 中均有表达，而 157 种蛋白质为阶段特异性蛋白，其中 31 种仅在 TT 中表达，126 种仅在 ST 中表达。功能注释显示 TT 和 ST 之间存在基本代谢的差异。TT 主要执行与基础代谢相关的功能，通过无性繁殖来实现生长和营养发育。相比之下，ST 执行更广泛的功能，包括大分子生物合成过程、基因表达和控制途径，这些功能可能与它的出芽/节段化、性分化和更复杂的生理功能有关。此外，生成的结果提供了大量的绦虫蛋白列表，这些蛋白对 M. corti 的功能研究很有意义。其中许多蛋白是新的候选诊断抗原，也是开发新型、更有效的抗寄生虫药物的潜在靶标。
绦虫病是一种严重影响人类和动物健康的寄生虫病。由于对绦虫生物学的许多方面仍知之甚少，包括这些寄生虫生命周期中发生的复杂发育过程，因此，开发更有效的诊断、治疗或控制绦虫病的策略受到了阻碍。Mesocestoides corti 是研究幼虫到成虫发育阶段（称为出芽）的良好实验模型，这一过程发生在典型的绦虫生命周期中。所进行的蛋白质组学方法提供了 M. corti 蛋白质的大规模鉴定和定量。在幼虫四叶期（TT）和有棘绦虫期（ST）中检测到许多阶段特异性或差异表达的蛋白质。对描述的蛋白质组成进行功能比较分析，揭示了与两个阶段特定特征相关的功能和过程，例如 TT 分化程度较低且为无性繁殖，而 ST 出芽/节段化和性分化。此外，许多鉴定出的阶段特异性蛋白可用作绦虫发育标记物，也是开发新型诊断方法和治疗绦虫病的治疗药物的潜在靶标。
