Chemical Engineering Department, School of Industrial Engineering - Centro de Investigación Tecnológico Industrial (MTI), University of Vigo, Campus As Lagoas-Marcosende, 36310 Vigo, Spain.
Chemical Engineering Department, Barcelona East School of Engineering (EEBE), Polytechnic University of Catalonia (UPC)-Barcelona TECH, Campus Diagonal-Besòs, 08930 Barcelona, Spain; Barcelona Research Center for Multiscale Science and Engineering, Campus Diagonal-Besòs, 08930 Barcelona, Spain.
J Pharm Sci. 2019 Jul;108(7):2296-2304. doi: 10.1016/j.xphs.2019.02.010. Epub 2019 Feb 21.
One of the most important challenges for pharmaceutical and cosmetic industries is solubilization and preservation of their active ingredients. Therefore, most of these formulations contain irritant chemical additives to improve their shelf-life and the solubility of hydrophobic ingredients. An interesting alternative to chemical surfactants and preservatives is the use of biosurfactants; thus, their surfactant properties and composition make them more biocompatible than their chemical counterparts. Moreover, some biosurfactants have shown antimicrobial activity in addition to their detergent capacity. In this work, the antimicrobial and irritant effect of 2 biosurfactant extracts was studied: one produced in a controlled fermentation process with Lactobacillus pentosus and the other produced from corn stream by spontaneous fermentation. The results showed a strong antimicrobial activity of the biosurfactant extract obtained from corn stream on pathogenic bacteria, in comparison with the L. pentosus biosurfactant extract. Moreover, both biosurfactants did not produce any irritant effect on the chorioallantoic membrane of hen's egg assay contrary to sodium dodecyl sulfate. This is the first study dealing with the application of biosurfactant extracts on sensitive biological membranes, and this is the first time that the preservative capacity of a biosurfactant extract obtained in spontaneous fermentation is being evaluated, achieving promising results.
对于制药和化妆品行业来说，最重要的挑战之一是其活性成分的增溶和保存。因此，这些制剂大多数都含有刺激性化学添加剂，以改善其保质期和疏水性成分的溶解度。化学表面活性剂和防腐剂的一个有趣替代品是生物表面活性剂的使用；因此，它们的表面活性剂特性和组成使它们比化学对应物更具有生物相容性。此外，一些生物表面活性剂除了具有去污能力外，还表现出抗菌活性。在这项工作中，研究了两种生物表面活性剂提取物的抗菌和刺激性作用：一种是通过控制发酵过程用戊糖乳杆菌生产的，另一种是通过玉米流自发发酵生产的。结果表明，与戊糖乳杆菌生物表面活性剂提取物相比，从玉米流中获得的生物表面活性剂提取物对病原菌具有很强的抗菌活性。此外，与十二烷基硫酸钠相反，两种生物表面活性剂在鸡胚绒毛尿囊膜的鸡胚绒毛尿囊膜试验中均未产生任何刺激性作用。这是首次研究生物表面活性剂提取物在敏感生物膜上的应用，也是首次评估自发发酵获得的生物表面活性剂提取物的防腐能力，取得了有希望的结果。
