Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Universitat Internacional de Catalunya, Barcelona, Spain; Department of Oral Surgery and Stomatology, School of Dental Medicine, University of Bern, Switzerland; Robert K. Schenk Laboratory of Oral Histology, School of Dental Medicine, University of Bern, Switzerland.
Department of Oral and Maxillofacial Surgery, School of Dental Medicine, Universitat Internacional de Catalunya, Barcelona, Spain.
Arch Oral Biol. 2019 Apr;100:57-68. doi: 10.1016/j.archoralbio.2019.02.006. Epub 2019 Feb 16.
Guided bone / tissue regeneration (GBR/GTR) procedures are necessary to improve conditions for implant placement. These techniques in turn can be enhanced by using growth factors (GFs) such as bone morphogenetic protein (BMP-2) and platelet-derived growth factor (PDGF) to accelerate regeneration. The aim of the present systematic review was to evaluate the GF loading and release kinetics of barrier membranes.
A total of 138 articles were screened in PubMed databases, and 31 meeting the inclusion criteria were included in the present systematic review.
All the articles evaluated bio-resorbable membranes, especially collagen or polymer-based membranes. In most studies, the retention and release kinetics of osteogenic GFs such as BMP-2 and PDGF were widely investigated. Growth factors were incorporated to the membranes by soaking and incubating the membranes in GF solution, followed by lyophilization, or mixing in the polymers before evaporation. Adsorption onto the membranes depended upon the membrane materials and additional reagents such as heparin, cross-linkers and GF concentration. Interestingly, most studies showed two phases of GF release from the membranes: a first phase comprising a burst release (about 1 day), followed by a second phase characterized by slower release. Furthermore, all the studies demonstrated the controlled release of sufficient concentrations of GFs from the membranes for bioactivities.
The adsorption and release kinetics varied among the different materials, forms and GFs. The combination of membrane materials, GFs and manufacturing methods should be considered for optimizing GBR/GTR procedures.
引导骨/组织再生（GBR/GTR）程序对于改善种植体植入条件是必要的。这些技术反过来可以通过使用生长因子（GFs）如骨形成蛋白（BMP-2）和血小板衍生生长因子（PDGF）来加速再生。本系统评价的目的是评估屏障膜的 GF 加载和释放动力学。
在 PubMed 数据库中总共筛选了 138 篇文章，纳入了 31 篇符合纳入标准的文章进行本系统评价。
所有评估的文章都评估了生物可吸收膜，特别是胶原或聚合物基膜。在大多数研究中，广泛研究了成骨 GFs 如 BMP-2 和 PDGF 的保留和释放动力学。生长因子通过将膜浸泡和孵育在 GF 溶液中，然后进行冻干，或在蒸发前将其混合在聚合物中，被掺入到膜中。吸附到膜上取决于膜材料和其他试剂如肝素、交联剂和 GF 浓度。有趣的是，大多数研究表明 GF 从膜中的释放存在两个阶段：第一阶段是突释（约 1 天），随后是第二阶段，其特征是释放较慢。此外，所有研究都证明了从膜中以足够的 GF 浓度持续释放以实现生物活性。
吸附和释放动力学因不同的材料、形式和 GFs 而异。应考虑膜材料、GFs 和制造方法的组合，以优化 GBR/GTR 程序。
