The First Affiliated Hospital, Jinan University, Guangzhou 510632, Guangdong, China; Formula-pattern Research Center, School of Traditional Chinese Medicine, Jinan University, Guangzhou 510632, Guangdong, China; Jiangmen Central Hospital, Affiliated Jiangmen Hospital of Sun Yat-sen University, Jiangmen 529030, China.
Integrated Chinese and Western Medicine Postdoctoral research station, Jinan University, Guangzhou 510632, China.
Biomed Pharmacother. 2019 Apr;112:108621. doi: 10.1016/j.biopha.2019.108621. Epub 2019 Feb 21.
Depression has become the leading cause of disability worldwide and a growing public health problem in China. In addition, intestinal flora may be associated with depression. This study investigated the effect of the decoction Xiaoyaosan (XYS) against depressive behavior through the regulation of intestinal flora. Fifty-two healthy male Sprague-Dawley rats were randomly divided into four groups (i.e., control, model, XYS, and fluoxetine). The latter three groups were subjected to 21 days of chronic restraint stress to produce the stress depression model. Rats in the XYS and fluoxetine groups received intragastric administration of XYS and fluoxetine, respectively. The behavioral changes of the rats were observed after 21 days. Stool specimens were sequenced using the 16S rDNA high-throughput method to detect the structure and changes in intestinal flora. There was no difference observed in alpha diversity among the groups. At the phylum level, XYS regulated the abundance of Bacteroidetes, Proteobacteria, Firmicutes, Chloroflexi, and Planctomycetes. At the genus level, XYS reduced the abundance of the Prevotellaceae_Ga6A1_group, Prevotellaceae_UCG-001, and Desulfovibrio. On the contrary, it increased the abundance of the Ruminococcaceae family to improve depression-like behavior. The mechanism involved in this process may be related to short-chain fatty acids, lipopolysaccharides, and intestinal inflammation.
抑郁症已成为全球范围内导致残疾的主要原因，也是中国日益严重的公共卫生问题。此外，肠道菌群可能与抑郁症有关。本研究通过调节肠道菌群，探讨了逍遥散（XYS）对抑郁行为的影响。52 只健康雄性 Sprague-Dawley 大鼠随机分为四组（即对照组、模型组、XYS 组和氟西汀组）。后三组进行 21 天慢性束缚应激，制作应激抑郁模型。XYS 组和氟西汀组大鼠分别给予灌胃 XYS 和氟西汀。21 天后观察大鼠的行为变化。采用 16S rDNA 高通量方法对粪便标本进行测序，检测肠道菌群的结构和变化。组间 alpha 多样性无差异。在门水平上，XYS 调节了拟杆菌门、变形菌门、厚壁菌门、绿弯菌门和浮霉菌门的丰度。在属水平上，XYS 降低了普雷沃氏菌科_Ga6A1 群、普雷沃氏菌科_UCG-001 和脱硫弧菌的丰度。相反，它增加了真细菌科家族的丰度，以改善抑郁样行为。这一过程涉及的机制可能与短链脂肪酸、脂多糖和肠道炎症有关。
