Malachowa Natalia, Kobayashi Scott D, Lovaglio Jamie, DeLeo Frank R
Laboratory of Bacteriology, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
Rocky Mountain Veterinary Branch, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT, USA.
Methods Mol Biol. 2019;1960:139-147. doi: 10.1007/978-1-4939-9167-9_12.
Bacterial skin and soft tissue infections are abundant worldwide, and many are caused by Staphylococcus aureus. Indeed, S. aureus is the leading cause of skin and soft tissue infections in the USA. Here we describe a mouse model of skin and soft tissue infection induced by subcutaneous inoculation of S. aureus. This animal model can be used to investigate a number of factors related to the pathogenesis of skin and soft tissue infections, including strain virulence and the contribution of specific bacterial molecules to disease, and it can be employed to test the potential effectiveness of antibiotic therapies or vaccine candidates.
细菌性皮肤和软组织感染在全球范围内很常见，许多感染是由金黄色葡萄球菌引起的。事实上，金黄色葡萄球菌是美国皮肤和软组织感染的主要原因。在这里，我们描述了一种通过皮下接种金黄色葡萄球菌诱导的皮肤和软组织感染小鼠模型。这种动物模型可用于研究与皮肤和软组织感染发病机制相关的许多因素，包括菌株毒力以及特定细菌分子对疾病的影响，并且可用于测试抗生素疗法或候选疫苗的潜在有效性。
