Ansari S, Nepal H P, Gautam R, Shrestha S, Chhetri M R, Chapagain M L
Department of Microbiology, Chitwan Medical College, Bharatpur, Chitwan, Nepal.
Department of Community Medicine and Public Health, Chitwan Medical College, Bharatpur, Chitwan, Nepal.
J Nepal Health Res Counc. 2015 May-Aug;13(30):126-32.
Staphylococcus aureus (S. aureus) colonizing the nares, is the leading cause of hospital as well as community acquired infections. The aim of this study was to identify the S. aureus from skin, soft tissue and bone related infections and typing them on the basis of antimicrobial susceptibility profile.
Clinical samples were collected from patients with skin, soft tissue and bone related infections from January to October 2013 at Chitwan Medical College Teaching Hospital, Bharatpur. The S. aureus, small colony variants and methicillin resistant strains were identified by standard microbiological methods recommended by American Society for Microbiology. Antimicrobial susceptibility testing was performed by modified Kirby-Bauer disk diffusion method.
Among 333 samples processed, there was positive growth of S. aureus in 66 (19.8%) samples. Among the isolated S. aureus, 10 isolates were small colony variants of S. aureus and all the strains recovered were thymidine independent in this study. Amikacin, vancomycin and teicoplanin were found to be the most effective antibiotics. Methicillin resistance was found in 34.8% isolates of S. aureus.
It can be concluded that, S. aureus is one of the causative agent of pyogenic infections and the trend of antibiotic resistant is alarmingly high and also the rate of methicillin resistant S. aureus is comparably high in this study.
鼻腔定植的金黄色葡萄球菌是医院获得性感染以及社区获得性感染的主要原因。本研究的目的是从皮肤、软组织和骨相关感染中鉴定金黄色葡萄球菌，并根据抗菌药物敏感性谱对其进行分型。
2013年1月至10月期间，从巴拉特普尔奇旺医学院教学医院收集患有皮肤、软组织和骨相关感染的患者的临床样本。金黄色葡萄球菌、小菌落变异株和耐甲氧西林菌株通过美国微生物学会推荐的标准微生物学方法进行鉴定。抗菌药物敏感性试验采用改良的 Kirby-Bauer 纸片扩散法进行。
在处理的333份样本中，66份（19.8%）样本中金黄色葡萄球菌生长呈阳性。在分离出的金黄色葡萄球菌中，有10株为金黄色葡萄球菌小菌落变异株，且本研究中所有回收菌株均不依赖胸苷。发现阿米卡星、万古霉素和替考拉宁是最有效的抗生素。在34.8%的金黄色葡萄球菌分离株中发现了耐甲氧西林现象。
可以得出结论，金黄色葡萄球菌是化脓性感染的病原体之一，抗生素耐药趋势惊人地高，并且在本研究中耐甲氧西林金黄色葡萄球菌的比例也相对较高。
