Yadav Umesh C S, Ramana Kota V
Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
Methods Mol Biol. 2019;1960:161-168. doi: 10.1007/978-1-4939-9167-9_14.
Uveitis can affect individuals of all ages, genders, and races and accounts for 10-15% of all cases of blindness. Uveitis represents a diverse array of intraocular inflammatory conditions that can be associated with complications from autoimmune diseases, bacterial infections, viral infections, chemical injuries, and metabolic issues. In rodents, endotoxin-induced uveitis (EIU) is an efficient experimental model to study pathological mechanisms associated with the disease and evaluate the pharmacological efficacy of potential new drug agents. In the EIU model, uveitis is characterized by clinically relevant inflammation, including inflammatory exudates and cells infiltrated into the anterior and vitreous eye chambers. EIU in small animal models, including rats, mice, and rabbits, is characterized by a short-lived uveal inflammation. This inflammation can be facilitated using bacterial endotoxins, such as lipopolysaccharide (LPS). In this chapter, we present a reliable, reproducible, and simplified protocol to induce EIU in mice. This method is flexible and can be applied for EIU induction in other small animals and rodents.
葡萄膜炎可影响所有年龄、性别和种族的个体，占所有失明病例的10%-15%。葡萄膜炎代表了一系列多样的眼内炎症性疾病，可能与自身免疫性疾病、细菌感染、病毒感染、化学损伤和代谢问题引发的并发症相关。在啮齿动物中，内毒素诱导的葡萄膜炎（EIU）是一种用于研究该疾病相关病理机制以及评估潜在新药药理疗效的有效实验模型。在EIU模型中，葡萄膜炎的特征是具有临床相关性的炎症，包括炎性渗出物以及浸润到眼前房和玻璃体腔的细胞。在包括大鼠、小鼠和兔子在内的小动物模型中，EIU的特征是葡萄膜炎症持续时间较短。使用细菌内毒素，如脂多糖（LPS），可促进这种炎症。在本章中，我们介绍一种在小鼠中诱导EIU的可靠、可重复且简化的方案。该方法具有灵活性，可应用于其他小动物和啮齿动物的EIU诱导。
