Palmer Lauren D, Green Erin R, Sheldon Jessica R, Skaar Eric P
Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Vanderbilt Institute for Infection, Immunology and Inflammation, Vanderbilt University Medical Center, Nashville, TN, USA.
Methods Mol Biol. 2019;1946:289-305. doi: 10.1007/978-1-4939-9118-1_26.
Acinetobacter baumannii is a Gram-negative opportunistic pathogen and a leading cause of ventilator-associated pneumonia. Murine models of A. baumannii lung infection allow researchers to experimentally assess A. baumannii virulence and host response. Intranasal administration of A. baumannii models acute lung infection. This chapter describes the methods to test A. baumannii virulence in a murine model of lung infection, including assessing the competitive index of a bacterial mutant and the associated inflammatory responses.
鲍曼不动杆菌是一种革兰氏阴性机会致病菌，也是呼吸机相关性肺炎的主要病因。鲍曼不动杆菌肺部感染的小鼠模型使研究人员能够通过实验评估鲍曼不动杆菌的毒力和宿主反应。经鼻给予鲍曼不动杆菌可模拟急性肺部感染。本章介绍了在小鼠肺部感染模型中测试鲍曼不动杆菌毒力的方法，包括评估细菌突变体的竞争指数和相关的炎症反应。
