Harris Greg, KuoLee Rhonda, Xu H Howard, Chen Wangxue
Human Health and Therapeutics, National Research Council Canada, Ottawa, Ontario, Canada.
Department of Biological Sciences, California State University, Los Angeles, California.
Curr Protoc Microbiol. 2017 Aug 11;46:6G.3.1-6G.3.23. doi: 10.1002/cpmc.36.
This unit describes basic protocols for infecting mice through intranasal and intraperitoneal routes with Acinetobacter baumannii to induce associated pneumonia and sepsis, the two most common manifestations of clinical infections with this pathogen. By selecting the appropriate protocols and bacterial strains of different virulence, these mouse models provide an opportunity to study the infection pathogenesis and host-immune responses, and to evaluate the efficacies of prophylactic and therapeutic anti-A. baumannii candidates. © 2017 by John Wiley & Sons, Inc.
本单元描述了通过鼻内和腹腔途径用鲍曼不动杆菌感染小鼠以诱导相关肺炎和败血症的基本方案，这是该病原体临床感染的两种最常见表现形式。通过选择合适的方案和不同毒力的细菌菌株，这些小鼠模型为研究感染发病机制和宿主免疫反应，以及评估预防性和治疗性抗鲍曼不动杆菌候选药物的疗效提供了机会。© 2017约翰威立父子公司。
