• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

与急性致死性肺炎发病机制潜在相关的肺固有免疫反应的转录组分析

Transcriptome Profiling of Lung Innate Immune Responses Potentially Associated With the Pathogenesis of Acute Lethal Pneumonia.

作者信息

Zeng Xi, Gu Hao, Peng Liusheng, Yang Yao, Wang Ning, Shi Yun, Zou Quanming

机构信息

Department of Microbiology and Biochemical Pharmacy, National Engineering Research Center of Immunological Products, College of Pharmacy, Army Medical University, Chongqing, China.

Institute of Biopharmaceutical Research, West China Hospital, Sichuan University, Chengdu, China.

出版信息

Front Immunol. 2020 Apr 22;11:708. doi: 10.3389/fimmu.2020.00708. eCollection 2020.

DOI:10.3389/fimmu.2020.00708
PMID:32391015
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7188829/
Abstract

is one of the dominating causes of nosocomial pneumonia, however, very little is known about the host immune response associated with pathogenesis of infection. Here, we used a hypervirulent to establish an acute lethal pneumonia, supported by high bacterial burdens, severe inflammatory cells infiltration and lung damage. The lung transcriptome changes in response to lethal pneumonia were detected by RNA sequencing. The results showed that 6,288 host genes changed expression, with 3,313 upregulated genes and 2,975 downregulated genes. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that genes related to TNF, cytokine-cytokine receptor interaction, Toll-like receptor, NOD-like receptor, NF-κB, Jak-STAT, HIF-1 signaling pathways, apoptosis, and phagosome were significantly upregulated. Whereas, genes associated with PI3K-AKT signaling pathway, glycolysis/gluconeogenesis, amino acid and fatty acid metabolism were downregulated. Immune cell typing highlighted the inflammatory response of innate immune cells headed by neutrophils. The reliability of RNA sequencing results were verified with selected differentially expressed genes by real-time PCR. This work provides an insight into the pathogenesis of lethal lung infection.

摘要

是医院获得性肺炎的主要病因之一，然而，关于与感染发病机制相关的宿主免疫反应却知之甚少。在此，我们使用一种高毒力菌株建立了急性致死性肺炎，其特征为高细菌载量、严重的炎性细胞浸润和肺损伤。通过RNA测序检测了致死性肺炎诱导的肺转录组变化。结果显示，6288个宿主基因发生表达变化，其中3313个基因上调，2975个基因下调。基因本体论和京都基因与基因组百科全书通路分析显示，与肿瘤坏死因子、细胞因子-细胞因子受体相互作用、Toll样受体、NOD样受体、核因子κB、Jak-STAT、低氧诱导因子-1信号通路、凋亡和吞噬体相关的基因显著上调。而与磷脂酰肌醇-3激酶-蛋白激酶B信号通路、糖酵解/糖异生、氨基酸和脂肪酸代谢相关的基因下调。免疫细胞分型突出了以中性粒细胞为首的固有免疫细胞的炎症反应。通过实时PCR对选定的差异表达基因验证了RNA测序结果的可靠性。这项工作为致死性肺部感染的发病机制提供了见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/876eeb8b59d1/fimmu-11-00708-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/346a5b0ac03e/fimmu-11-00708-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/da5e2e839ffa/fimmu-11-00708-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/f15509be0484/fimmu-11-00708-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/14b19dc81c86/fimmu-11-00708-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/3ce8eaf828f9/fimmu-11-00708-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/3d784127866e/fimmu-11-00708-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/876eeb8b59d1/fimmu-11-00708-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/346a5b0ac03e/fimmu-11-00708-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/da5e2e839ffa/fimmu-11-00708-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/f15509be0484/fimmu-11-00708-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/14b19dc81c86/fimmu-11-00708-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/3ce8eaf828f9/fimmu-11-00708-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/3d784127866e/fimmu-11-00708-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d083/7188829/876eeb8b59d1/fimmu-11-00708-g0007.jpg

相似文献

1
Transcriptome Profiling of Lung Innate Immune Responses Potentially Associated With the Pathogenesis of Acute Lethal Pneumonia.与急性致死性肺炎发病机制潜在相关的肺固有免疫反应的转录组分析
Front Immunol. 2020 Apr 22;11:708. doi: 10.3389/fimmu.2020.00708. eCollection 2020.
2
A lethal pneumonia model of Acinetobacter baumannii: an investigation in immunocompetent mice.鲍曼不动杆菌致死性肺炎模型：在免疫功能正常小鼠中的研究。
Clin Microbiol Infect. 2019 Apr;25(4):516.e1-516.e4. doi: 10.1016/j.cmi.2018.12.020. Epub 2018 Dec 22.
3
Analysis of Immune Responses in -Infected Klotho Knockout Mice: A Mouse Model of Infection in Aged Hosts.Klotho 敲除小鼠中的免疫应答分析：衰老宿主中 感染的小鼠模型。
Front Immunol. 2020 Nov 23;11:601614. doi: 10.3389/fimmu.2020.601614. eCollection 2020.
4
Toll-Like Receptor 9 Contributes to Defense against Acinetobacter baumannii Infection.Toll样受体9有助于抵御鲍曼不动杆菌感染。
Infect Immun. 2015 Oct;83(10):4134-41. doi: 10.1128/IAI.00410-15. Epub 2015 Aug 3.
5
The acute-phase response and serum amyloid A inhibit the inflammatory response to Acinetobacter baumannii Pneumonia.急性期反应和血清淀粉样蛋白A可抑制对鲍曼不动杆菌肺炎的炎症反应。
J Infect Dis. 2006 Jan 15;193(2):187-95. doi: 10.1086/498876. Epub 2005 Dec 7.
6
Broad-spectrum suppression of bacterial pneumonia by aminoglycoside-propagated Acinetobacter baumannii.氨基糖苷类传播的鲍曼不动杆菌广谱抑制细菌性肺炎。
PLoS Pathog. 2020 Mar 13;16(3):e1008374. doi: 10.1371/journal.ppat.1008374. eCollection 2020 Mar.
7
High susceptibility to respiratory Acinetobacter baumannii infection in A/J mice is associated with a delay in early pulmonary recruitment of neutrophils.A/J小鼠对呼吸道鲍曼不动杆菌感染的高易感性与中性粒细胞早期肺募集延迟有关。
Microbes Infect. 2009 Oct;11(12):946-55. doi: 10.1016/j.micinf.2009.06.003. Epub 2009 Jun 30.
8
Differential roles of CD14 and toll-like receptors 4 and 2 in murine Acinetobacter pneumonia.CD14及Toll样受体4和2在小鼠不动杆菌肺炎中的不同作用
Am J Respir Crit Care Med. 2006 Jan 1;173(1):122-9. doi: 10.1164/rccm.200505-730OC. Epub 2005 Oct 6.
9
Assessing Acinetobacter baumannii Virulence and Persistence in a Murine Model of Lung Infection.在肺部感染小鼠模型中评估鲍曼不动杆菌的毒力和持续性
Methods Mol Biol. 2019;1946:289-305. doi: 10.1007/978-1-4939-9118-1_26.
10
A mouse model of Acinetobacter baumannii-associated pneumonia using a clinically isolated hypervirulent strain.使用一株临床分离的高毒力菌株建立鲍曼不动杆菌相关性肺炎的小鼠模型。
Antimicrob Agents Chemother. 2013 Aug;57(8):3601-13. doi: 10.1128/AAC.00944-13. Epub 2013 May 20.

引用本文的文献

1
Goblet cell breakdown: transcriptomics reveals Acinetobacter baumannii early and robust inflammatory response in differentiated human bronchial epithelial cells.杯状细胞破坏：转录组学揭示鲍曼不动杆菌在分化的人支气管上皮细胞中引发的早期且强烈的炎症反应。
J Biomed Sci. 2025 Jul 9;32(1):63. doi: 10.1186/s12929-025-01159-1.
2
Hypervirulent (hvAB): The Convergence of Virulence and Multidrug Resistance.高毒力（hvAB）：毒力与多重耐药性的融合
Antibiotics (Basel). 2025 May 29;14(6):551. doi: 10.3390/antibiotics14060551.
3
CCL28 modulates neutrophil responses during infection with mucosal pathogens.

本文引用的文献

1
Tug of war between Acinetobacter baumannii and host immune responses.鲍曼不动杆菌与宿主免疫反应的拔河比赛。
Pathog Dis. 2018 Dec 1;76(9). doi: 10.1093/femspd/ftz004.
2
A lethal pneumonia model of Acinetobacter baumannii: an investigation in immunocompetent mice.鲍曼不动杆菌致死性肺炎模型：在免疫功能正常小鼠中的研究。
Clin Microbiol Infect. 2019 Apr;25(4):516.e1-516.e4. doi: 10.1016/j.cmi.2018.12.020. Epub 2018 Dec 22.
3
Metabolic regulation of infection and inflammation.感染和炎症的代谢调控。
CCL28 调节黏膜病原体感染期间的中性粒细胞反应。
Elife. 2024 Aug 28;13:e78206. doi: 10.7554/eLife.78206.
4
efficacy of phage cocktails against carbapenem resistance in the rat pneumonia model.噬菌体鸡尾酒对大鼠肺炎模型中碳青霉烯耐药性的疗效。
J Virol. 2024 Jul 23;98(7):e0046724. doi: 10.1128/jvi.00467-24. Epub 2024 Jun 12.
5
Antibacterial activity and mechanism of X33 antimicrobial oligopeptide against .X33抗菌寡肽对……的抗菌活性及作用机制
Synth Syst Biotechnol. 2024 Mar 15;9(2):312-321. doi: 10.1016/j.synbio.2024.03.002. eCollection 2024 Jun.
6
Giardia VSPAS7 protein attenuates Giardia intestinalis-induced host macrophage pyroptosis.贾第虫 VSPAS7 蛋白减轻贾第虫诱导的宿主巨噬细胞焦亡。
Parasit Vectors. 2023 Oct 11;16(1):359. doi: 10.1186/s13071-023-05949-0.
7
Host A-to-I RNA editing signatures in intracellular bacterial and single-strand RNA viral infections.细胞内细菌和单链 RNA 病毒感染中的 A-to-I RNA 编辑特征。
Front Immunol. 2023 Apr 4;14:1121096. doi: 10.3389/fimmu.2023.1121096. eCollection 2023.
8
Acinetobacter baumannii reinforces the pathogenesis by promoting IL-17 production in a mouse pneumonia model.在小鼠肺炎模型中，鲍曼不动杆菌通过促进白细胞介素-17的产生来增强发病机制。
Med Microbiol Immunol. 2023 Feb;212(1):65-73. doi: 10.1007/s00430-022-00757-2. Epub 2022 Dec 3.
9
Outer Membrane Protein A Induces Pulmonary Epithelial Barrier Dysfunction and Bacterial Translocation Through The TLR2/IQGAP1 Axis.外膜蛋白 A 通过 TLR2/IQGAP1 轴诱导肺上皮屏障功能障碍和细菌易位。
Front Immunol. 2022 Jun 30;13:927955. doi: 10.3389/fimmu.2022.927955. eCollection 2022.
10
and Its Secreted PPIB Trigger Inflammasome Activation and Pyroptosis in Macrophages through TLR4-Induced ROS Signaling and A20-Mediated NLRP3 Deubiquitination.并通过 TLR4 诱导的 ROS 信号和 A20 介导的 NLRP3 去泛素化来触发巨噬细胞中的炎症小体激活和细胞焦亡。
Cells. 2021 Dec 6;10(12):3425. doi: 10.3390/cells10123425.
Cytokine. 2018 Dec;112:1-11. doi: 10.1016/j.cyto.2018.11.016. Epub 2018 Nov 22.
4
Effect of Hypoxia on the Pathogenesis of Acinetobacter baumannii and Pseudomonas aeruginosa In Vitro and in Murine Experimental Models of Infection.缺氧对鲍曼不动杆菌和铜绿假单胞菌体外发病机制及感染小鼠模型的影响。
Infect Immun. 2018 Sep 21;86(10). doi: 10.1128/IAI.00543-18. Print 2018 Oct.
5
Aging exacerbates mortality of pneumonia and reduces the efficacies of antibiotics and vaccine.衰老会加剧肺炎导致的死亡率，并降低抗生素和疫苗的疗效。
Aging (Albany NY). 2018 Jul 18;10(7):1597-1608. doi: 10.18632/aging.101495.
6
Type I IFN operates pyroptosis and necroptosis during multidrug-resistant A. baumannii infection.I 型干扰素在多重耐药鲍曼不动杆菌感染期间作用于细胞焦亡和坏死性凋亡。
Cell Death Differ. 2018 Jul;25(7):1304-1318. doi: 10.1038/s41418-017-0041-z. Epub 2018 Jan 19.
7
Nod2 is required for the early innate immune clearance of Acinetobacter baumannii from the lungs.Nod2 对于早期固有免疫清除肺部鲍曼不动杆菌是必需的。
Sci Rep. 2017 Dec 12;7(1):17429. doi: 10.1038/s41598-017-17653-y.
8
Virulence profiles and innate immune responses against highly lethal, multidrug-resistant nosocomial isolates of Acinetobacter baumannii from a tertiary care hospital in Mexico.来自墨西哥一家三级护理医院的高致死性、多重耐药鲍曼不动杆菌医院分离株的毒力特征及固有免疫反应
PLoS One. 2017 Aug 10;12(8):e0182899. doi: 10.1371/journal.pone.0182899. eCollection 2017.
9
NLRP3 inflammasome pathway has a critical role in the host immunity against clinically relevant Acinetobacter baumannii pulmonary infection.NLRP3 炎性小体通路在宿主对临床相关鲍曼不动杆菌肺部感染的免疫反应中起着关键作用。
Mucosal Immunol. 2018 Jan;11(1):257-272. doi: 10.1038/mi.2017.50. Epub 2017 Jun 14.
10
NLRP3 inflammasome mediates interleukin-1β production in immune cells in response to Acinetobacter baumannii and contributes to pulmonary inflammation in mice.NLRP3炎性小体介导免疫细胞对鲍曼不动杆菌产生白细胞介素-1β，并导致小鼠肺部炎症。
Immunology. 2017 Apr;150(4):495-505. doi: 10.1111/imm.12704. Epub 2017 Jan 30.