丝裂霉素C对正常细胞和范科尼贫血细胞中DNA合成速率的影响。

Effects of mitomycin C on the rate of DNA synthesis in normal and Fanconi anaemia cells.

作者信息

Claassen E, Kortbeek H, Arwert F

出版信息

Mutat Res. 1986 Jan;165(1):15-9. doi: 10.1016/0167-8817(86)90004-0.

Abstract

The effect of low doses mitomycin C (MMC) on DNA synthesis of fibroblast cell lines derived from normal individuals or patients with Fanconi anaemia (FA) was studied. Using low doses of MMC (12 ng/ml), little or no effect was observed on DNA synthesis of normal cells, whereas DNA synthesis of FA cells was greatly inhibited 24 and 48 h after treatment. This effect was due to a decrease in the number of DNA-synthesizing cells, while the amount of radioactivity incorporated per cell (as measured with grain counting in autoradiograms) remained the same. These findings indicate that the inhibition of semiconservative DNA synthesis induced by MMC in FA cells is not due to an inhibitory effect of unrepaired lesions on the rate of DNA synthesis but rather to a block in cell cycle progression.

摘要

研究了低剂量丝裂霉素C（MMC）对源自正常个体或范可尼贫血（FA）患者的成纤维细胞系DNA合成的影响。使用低剂量的MMC（12 ng/ml）时，未观察到对正常细胞DNA合成有明显影响，而在处理后24小时和48小时，FA细胞的DNA合成受到极大抑制。这种效应是由于DNA合成细胞数量减少，而每个细胞掺入的放射性量（通过放射自显影片中的颗粒计数测量）保持不变。这些发现表明，MMC在FA细胞中诱导的半保留DNA合成抑制不是由于未修复损伤对DNA合成速率的抑制作用，而是由于细胞周期进程的阻滞。