Nakazawa Takehito, Morimoto Ryota, Wu Hongli, Kodera Rina, Sakamoto Masahiro, Honda Yoichi
Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.
Fungal Biol. 2019 Mar;123(3):209-217. doi: 10.1016/j.funbio.2018.12.007. Epub 2018 Dec 27.
In nature, white-rot fungi efficiently degrade lignin present in wood biomass. Elucidation of molecular mechanisms underlying wood lignin biodegradation by white-rot fungi would contribute to the development of efficient and ecofriendly methods of producing valuable chemical products from wood biomass. Here, using forward genetics approach, we demonstrate that the mutant of a putative transcription factor gene, gat1-1, significantly decreases the ligninolytic activity of the white-rot fungus Pleurotus ostreatus, when grown on beech wood sawdust medium. We also show that this phenotype is dominant. In Schizophyllum commune, Gat1 was previously shown to be involved in fruiting body development. In this study, we reveal that the mutations in gat1 gene cause defects in fruiting body development in P. ostreatus. Unlike the previously reported recessive gene mutations that decrease the ligninolytic activity of P. ostreatus, the gat1-1 mutation and Δgat1 are dominant and would thus be useful for future studies on the functional role of the orthologs in other white-rot fungi.
在自然界中，白腐真菌能有效降解木材生物质中的木质素。阐明白腐真菌对木材木质素生物降解的分子机制，将有助于开发高效且环保的从木材生物质中生产有价值化学产品的方法。在此，我们采用正向遗传学方法证明，在以山毛榉木屑为培养基生长时，一个假定转录因子基因gat1 - 1的突变体显著降低了白腐真菌糙皮侧耳的木质素分解活性。我们还表明这种表型是显性的。在裂褶菌中，Gat1先前被证明参与子实体发育。在本研究中，我们揭示gat1基因突变会导致糙皮侧耳子实体发育缺陷。与先前报道的降低糙皮侧耳木质素分解活性的隐性基因突变不同，gat1 - 1突变和Δgat1是显性的，因此将有助于未来对其他白腐真菌中直系同源基因功能作用的研究。
