Wang Can, Dong Xiaoying, Wei Limu, Sun Junfeng, Zhao Fali, Meng Choushuan, Wu Dongdong, Wang Ting, Fu Lu
Laboratory of Cardiovascular Medicine, Department of Internal Medicine, First Affiliated Hospital, Harbin Medical University.
Int Heart J. 2019 Mar 20;60(2):384-391. doi: 10.1536/ihj.18-131. Epub 2019 Feb 22.
The physiological control of appetite regulation involves circulating hormones with orexigenic (ghrelin) and anorexigenic (cholecystokinin) properties that induce alterations in energy intake via perceptions of hunger and satiety. We sought to investigate the relationship between appetite-regulating hormones and the cachexia associated with chronic heart failure.We randomized male Sprague-Dawley rats into myocardial infarction (MI) or sham operation (SO) groups. The levels of brain natriuretic peptide (BNP), cholecystokinin (CCK) and ghrelin in the plasma of all rats were detected by enzyme-linked immunosorbent assay (ELISA); the expression of BNP, CCK, and ghrelin in the myocardial tissue of all rats were detected by western blotting, immunohistochemistry, real-time polymerase chain reaction (PCR); myocardial morphology was assessed by microscopy.Plasma BNP and CCK levels in the cardiac cachexia (CC) groups and the heart failure non-cachexia (HF-nc) groups were significantly higher than those in the control groups (P < 0.01), and the expression of BNP and CCK in the myocardial tissue of rats: in CC groups and HF-nc groups were increased compared with the corresponding control groups (P < 0.01). In contrast, Plasma and cardiac expression of ghrelin decreased compared with the sham group (P < 0.01). Furthermore, plasma CCK levels were positively correlated with BNP concentrations (P < 0.001) and significantly negatively correlated with the ejection fraction (P < 0.001) in model animals; plasma ghrelin levels were negatively associated with BNP levels (P = 0.0023) and positively associated with ejection fraction (P = 0.0042).The appetite-regulating hormones (ghrelin and CCK) may present as a potential significant biomarker for cachexia associated with chronic heart failure.
食欲调节的生理控制涉及具有促食欲（胃饥饿素）和抑食欲（胆囊收缩素）特性的循环激素，这些激素通过饥饿和饱腹感的感知来诱导能量摄入的改变。我们试图研究食欲调节激素与慢性心力衰竭相关恶病质之间的关系。我们将雄性Sprague-Dawley大鼠随机分为心肌梗死（MI）组或假手术（SO）组。采用酶联免疫吸附测定（ELISA）法检测所有大鼠血浆中脑钠肽（BNP）、胆囊收缩素（CCK）和胃饥饿素的水平；采用蛋白质印迹法、免疫组织化学法、实时聚合酶链反应（PCR）检测所有大鼠心肌组织中BNP、CCK和胃饥饿素的表达；通过显微镜评估心肌形态。心脏恶病质（CC）组和心力衰竭非恶病质（HF-nc）组的血浆BNP和CCK水平显著高于对照组（P<0.01），大鼠心肌组织中BNP和CCK的表达：CC组和HF-nc组与相应对照组相比增加（P<0.01）。相比之下，与假手术组相比，胃饥饿素的血浆和心脏表达降低（P<0.01）。此外，在模型动物中，血浆CCK水平与BNP浓度呈正相关（P<0.001），与射血分数呈显著负相关（P<0.001）；血浆胃饥饿素水平与BNP水平呈负相关（P = 0.0023），与射血分数呈正相关（P = 0.0042）。食欲调节激素（胃饥饿素和CCK）可能是慢性心力衰竭相关恶病质的潜在重要生物标志物。
