Klessen C
J Histochem Cytochem. 1978 Sep;26(9):759-60. doi: 10.1177/26.9.30800.
A cytochemical method is presented for the demonstration of proteases in human polymorphonuclear (PMN) neutrophils on fixed blood smears. This new technique is based on solubilization of proteases from PMN neutrophils by incubation with 0.25 M NaCl in borate buffer at pH 8.5 which leads to degradation of erythrocytes and plasma in a disclike zone (halo) around centrally situated PMN neutrophils, an effect that is visualized by staining smears using a modified colloidal iron reaction. Halo formation is inhibited by trypsin inhibitors of soya-bean as well as of chicken egg white mucoid and by phenylmethyl-sulfonylfluoride.
本文介绍了一种用于在固定血涂片上显示人多形核（PMN）中性粒细胞中蛋白酶的细胞化学方法。这项新技术基于在pH 8.5的硼酸盐缓冲液中用0.25 M NaCl孵育，使PMN中性粒细胞中的蛋白酶溶解，这会导致位于中央的PMN中性粒细胞周围的圆盘状区域（晕圈）中的红细胞和血浆降解，通过使用改良的胶体铁反应对涂片进行染色来观察这种效应。大豆和鸡卵类粘蛋白的胰蛋白酶抑制剂以及苯甲基磺酰氟可抑制晕圈形成。
