Klessen C, Tekolf W
Histochemistry. 1980;69(3):307-14. doi: 10.1007/BF00489776.
Neutral proteases can be released from PMN neutrophils in blood smears from healthy subjects by incubation with NaCl-borate buffer. The activity of the PMN proteases can be revealed by the degradation of erythrocytes and plasma within ring-shaped areas centered around each neutrophil (halo effect). During the acute stage of various inflammatory diseases (pneumonia, meningitis, cholecystitis, etc.) the activity of neutral PMN proteases is substantially reduced, as reflected by reduced halo formation. After recovery, halo formation returns to normal. Temporary lowering of neutral PMN proteases is thus one of a series of functional defects of PMN neutrophils which are detectable in the course of acute infectious diseases. These include reduced phagocytosis, altered chemotaxis and reduced bactericidal function. The cytochemical test for neutrophilic granulocyte function used in the present investigation is especially practical by comparison with the other techniques: it saves time and is simple to perform.
通过与氯化钠 - 硼酸盐缓冲液孵育，可从健康受试者血涂片的PMN中性粒细胞中释放中性蛋白酶。PMN蛋白酶的活性可通过以每个中性粒细胞为中心的环形区域内红细胞和血浆的降解来揭示（晕轮效应）。在各种炎症性疾病（肺炎、脑膜炎、胆囊炎等）的急性期，中性PMN蛋白酶的活性显著降低，这表现为晕轮形成减少。恢复后，晕轮形成恢复正常。因此，中性PMN蛋白酶的暂时降低是急性传染病过程中可检测到的PMN中性粒细胞一系列功能缺陷之一。这些缺陷包括吞噬作用降低、趋化性改变和杀菌功能降低。与其他技术相比，本研究中使用的嗜中性粒细胞功能的细胞化学测试特别实用：它节省时间且操作简单。
