介观光学投影断层扫描中时间域和角度域散射抑制的比较：一项模拟研究
Comparison of time- and angular-domain scatter rejection in mesoscopic optical projection tomography: a simulation study.
作者信息
Sinha L, Massanes F, Torres V C, Li C, Tichauer K M, Brankov J G
机构信息
Biomedical Engineering, Illinois Institute of Technology, 3255 South Dearborn Street, Chicago, IL 60616, USA.
Electrical and Computer Engineering, Illinois Institute of Technology, 3301 South Dearborn Street, Chicago, IL 60616, USA.
出版信息
Biomed Opt Express. 2019 Jan 23;10(2):747-760. doi: 10.1364/BOE.10.000747. eCollection 2019 Feb 1.
Optical imaging offers exquisite sensitivity and resolution for assessing biological tissue in microscopy applications; however, for samples that are greater than a few hundred microns in thickness (such as whole tissue biopsies), spatial resolution is substantially limited by the effects of light scattering. To improve resolution, time- and angular-domain methods have been developed to reject detection of highly scattered light. This work utilizes a modified version of a commonly used Monte Carlo light propagation software package (MCML) to present the first comparison of time- and angular-domain improvements in spatial resolution with respect to varying sample thickness and optical properties (absorption and scattering). Specific comparisons were made at various tissue thicknesses (1-6 mm) assuming either typical (average) soft tissue scattering properties, ' = 10 cm, or low scattering properties, ' = 3.4 cm, as measured in lymph nodes.
光学成像在显微镜应用中为评估生物组织提供了极高的灵敏度和分辨率；然而，对于厚度超过几百微米的样本（如全组织活检样本），空间分辨率会受到光散射效应的显著限制。为了提高分辨率，人们开发了时域和角域方法来排除对高度散射光的检测。这项工作利用了一个常用的蒙特卡罗光传播软件包（MCML）的修改版本，首次比较了时域和角域在空间分辨率方面随样本厚度和光学特性（吸收和散射）变化的改进情况。在各种组织厚度（1 - 6毫米）下进行了具体比较，假设具有典型（平均）软组织散射特性，μ' = 10厘米，或低散射特性，μ' = 3.4厘米，这是在淋巴结中测量得到的。
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