National Key Discipline Laboratory, Department of Nutrition and Food Hygiene, School of Public Health, Harbin Medical University, Harbin, P. R. China.
Food Funct. 2019 Mar 20;10(3):1552-1562. doi: 10.1039/c8fo02321b.
The aim of our work was to use a targeted GC-MS approach to investigate the difference in organic acid profiles between individuals with impaired fasting glucose (IFG) and healthy controls and then to investigate the alterations in postprandial organic acid profiles after a meal of highland barley (HB) in IFG individuals. Firstly, 30 IFG and 30 healthy individuals were recruited and 26 organic acids were detected to characterize the organic acid profiles in the fasting serum metabolome. Secondly, 15 participants of the IFG group received three different loads: glucose (GL), white rice (WR) and HB. Serum was collected at time zero, 30, 60, 90 and 120 min after the test load. The results showed fasting organic acid profiles were different between the IFG group and the controls. For the postprandial changes in organic acids after the three test loads, six organic acids related to the tricarboxylic acid (TCA) cycle, namely citrate, cis-aconitic acid, fumarate, succinate, pyruvate and malate, had a significant test load effect (p < 0.01) and a significant time × test load interaction effect (p < 0.01). The AUC0-120 min values for citrate, fumarate and malate after WR and HB loads were significantly lower (p < 0.05) compared to the GL load. In addition, the AUC0-120 min value for pyroglutamic acid after WR and HB loads was significantly higher (p < 0.05) compared to the GL load, whereas the AUC0-120 min for malonic acid after WR and HB loads was significantly lower (p < 0.05) compared to the GL load. Altogether, these findings suggest that the HB load producing low postprandial glucose and insulin responses brings about several alterations in organic acids.
我们的工作目的是使用靶向 GC-MS 方法来研究空腹血糖受损（IFG）个体与健康对照者之间有机酸谱的差异，然后研究 IFG 个体餐后高海拔青稞（HB）餐后有机酸谱的变化。首先，招募了 30 名 IFG 患者和 30 名健康对照者，检测了 26 种有机酸以表征空腹血清代谢组中的有机酸谱。其次，IFG 组的 15 名参与者接受了三种不同的负荷：葡萄糖（GL）、白米（WR）和 HB。在测试负荷后 0、30、60、90 和 120 分钟采集血清。结果表明，IFG 组与对照组之间的空腹有机酸谱存在差异。对于三种测试负荷后有机酸的餐后变化，与三羧酸（TCA）循环相关的六种有机酸，即柠檬酸、顺乌头酸、富马酸、琥珀酸、丙酮酸和苹果酸，具有显著的测试负荷效应（p<0.01）和时间×测试负荷相互作用效应（p<0.01）。WR 和 HB 负荷后柠檬酸、富马酸和苹果酸的 AUC0-120min 值明显低于 GL 负荷（p<0.05）。此外，WR 和 HB 负荷后吡咯烷酮酸的 AUC0-120min 值明显高于 GL 负荷（p<0.05），而 WR 和 HB 负荷后丙二酸的 AUC0-120min 值明显低于 GL 负荷（p<0.05）。总之，这些发现表明 HB 负荷产生的低餐后血糖和胰岛素反应会引起几种有机酸的变化。
