Li C Y, Witzig T E, Phyliky R L, Ziesmer S C, Yam L T
Cancer. 1986 Feb 15;57(4):737-44. doi: 10.1002/1097-0142(19860215)57:4<737::aid-cncr2820570410>3.0.co;2-6.
Diagnosis of central nervous system (CNS) non-Hodgkin's lymphomas may be difficult despite the use of sophisticated scans and routine cytologic methods. The use of an immunoalkaline phosphatase technique to examine cerebrospinal fluid (CSF) containing many mononuclear cells is described. Monoclonal proliferations of B-lymphocytes were demonstrated in six patients with neurologic abnormalities, whose clinical findings and subsequent clinical courses were those of lymphoma. The diagnosis of CNS lymphoma could not be made, despite multiple diagnostic procedures, until the immunocytochemical studies were performed. In three other patients, a lymphoproliferative disorder was suspected; however, examination of CSF showed many T-lymphocytes but no monoclonal B-lymphocytes, consistent with a reactive lymphocytosis. The subsequent clinical courses of these patients have shown no evidence of CNS lymphoma. Immunocytochemical studies of CSF lymphocytes are useful in differentiating benign from malignant proliferations.
尽管使用了精密扫描和常规细胞学方法，中枢神经系统（CNS）非霍奇金淋巴瘤的诊断仍可能存在困难。本文描述了一种免疫碱性磷酸酶技术，用于检测含有许多单核细胞的脑脊液（CSF）。在6例有神经学异常的患者中证实了B淋巴细胞的单克隆增殖，其临床发现及随后的临床病程均符合淋巴瘤表现。在进行免疫细胞化学研究之前，尽管进行了多项诊断程序，仍无法确诊CNS淋巴瘤。在另外3例患者中，怀疑存在淋巴细胞增殖性疾病；然而，脑脊液检查显示有许多T淋巴细胞，但无单克隆B淋巴细胞，符合反应性淋巴细胞增多症。这些患者随后的临床病程未显示CNS淋巴瘤的证据。脑脊液淋巴细胞的免疫细胞化学研究有助于区分良性与恶性增殖。
