Gustafsson M K, Lehtonen M A, Sundler F
Cell Tissue Res. 1986;243(1):41-9. doi: 10.1007/BF00221850.
In the nervous system of the obligatory endoparasite Diphyllobothrium dendriticum immunoreactivity (IR) to growth hormone-releasing factor (GRF), peptide histidine isoleucine (PHI), bovine pancreatic polypeptide (BPP), gastrin, gastrin-releasing peptide (GRP), oxytocin, FMRFamide (FMRF) and serotonin (5HT) was demonstrated by immunocytochemical methods. A very strong GRF-IR was observed in the CNS and PNS of larvae and of the constantly growing adult worms. GRF-IR axon terminals occur beneath the basal lamina of the tegument along the inside of the bothridia, the holdfast organ of the worm. GRF-IR fibres surround the yolk producing vitelline glands and occur in the wall of the vagina. PHI-IR was observed in the CNS and PNS of larvae and adult worms. PHI-IR terminals occur beneath the basal lamina of the tegument along the strobila, the nutrient absorbing surface of the worm. PHI-IR fibres seem to innervate the testicular follicles. FMRF-IR fibres and perikarya occur close to the vitelline glands and the uterine pore and in the male copulatory organ. Numerous large 5HT-IR perikarya with long varicose fibres were observed in the nervous system of the worm. 5HT-IR perikarya occur close to the genital atrium. D. dendriticum is the phylogenetically lowest organism in which IR to PHI has been demonstrated.
采用免疫细胞化学方法，在专性内寄生虫阔节裂头绦虫的神经系统中，证实了其对生长激素释放因子（GRF）、肽组氨酸异亮氨酸（PHI）、牛胰多肽（BPP）、胃泌素、胃泌素释放肽（GRP）、催产素、FMRF酰胺（FMRF）和5-羟色胺（5HT）具有免疫反应性（IR）。在幼虫和不断生长的成虫的中枢神经系统和外周神经系统中，均观察到非常强烈的GRF免疫反应性。GRF免疫反应性轴突终末沿虫体的吸着器官——吸槽内部，出现在皮层基膜下方。GRF免疫反应性纤维围绕着产生卵黄的卵黄腺，并出现在阴道壁中。在幼虫和成虫的中枢神经系统和外周神经系统中，均观察到PHI免疫反应性。PHI免疫反应性终末沿虫体的节片链（即虫体的营养吸收表面），出现在皮层基膜下方。PHI免疫反应性纤维似乎支配着睾丸滤泡。FMRF免疫反应性纤维和神经周体靠近卵黄腺和子宫孔以及雄性交配器官。在该蠕虫的神经系统中，观察到许多带有长曲张纤维的大型5HT免疫反应性神经周体。5HT免疫反应性神经周体靠近生殖腔。阔节裂头绦虫是已证实对PHI具有免疫反应性的系统发育地位最低的生物。
