Fairweather I, McMullan M T, Johnston C F, Rogan M T, Hanna R E
School of Biology and Biochemistry, Queen's University of Belfast, Medical Biology Centre, Northern Ireland.
Parasitol Res. 1994;80(8):649-56. doi: 10.1007/BF00932948.
The localisation and distribution of 5-hydroxytryptamine (5-HT, or serotonin) and neuropeptides in the nervous system of the protoscolex of the hydatid organism Echinococcus granulosus were determined by an indirect immunofluorescence technique. Nerve-cell bodies immunoreactive for 5-HT occurred in the lateral ganglia and in association with the lateral longitudinal nerve cords. 5-HT immunostaining was also evident in the central nerve ring, in the rostellar nerves and in the nerve plexus innervating the suckers. Of the antisera used to screen the protoscolex for neuropeptide immunoreactivity (IR), immunostaining was obtained with those raised against pancreatic polypeptide (PP), peptide YY (PYY), substance P (SP), peptide histidine isoleucine (PHI) and vasoactive intestinal peptide (VIP). The most extensive pattern of IR occurred with antisera to PP and PYY. Immunoreactive nerve elements were evident in the lateral ganglia, central nerve ring, rostellar nerves, rostellar ganglia, sucker plexus and longitudinal nerve cords. The distribution of SP-, PHI- and VIP-IRs was more restricted: SP-IR occurred in the lateral ganglia and sucker nerves, whilst PHI- and VIP-immunoreactive nerve elements were associated with the lateral longitudinal nerve cords. Protoscoleces cultured in vitro for 29 days were also examined and neuroanatomical changes noted. A greater development of the longitudinal nerve cords and their cross-connectives in the body of the worm was evident, and a group of nerve cells were seen to develop at the posterior end of the main lateral nerve cords.
采用间接免疫荧光技术确定了细粒棘球绦虫幼虫原头蚴神经系统中5-羟色胺（5-HT，或血清素）和神经肽的定位与分布。对5-HT呈免疫反应的神经细胞体出现在外侧神经节，并与外侧纵神经索相连。在中枢神经环、顶突神经和支配吸盘的神经丛中也可见5-HT免疫染色。在用抗血清筛选原头蚴神经肽免疫反应性（IR）时，使用针对胰多肽（PP）、肽YY（PYY）、P物质（SP）、肽组氨酸异亮氨酸（PHI）和血管活性肠肽（VIP）的抗血清获得了免疫染色。IR最广泛的模式出现在针对PP和PYY的抗血清中。免疫反应性神经成分在外侧神经节、中枢神经环、顶突神经、顶突神经节、吸盘神经丛和纵神经索中明显可见。SP-、PHI-和VIP-IR的分布更局限：SP-IR出现在外侧神经节和吸盘神经中，而PHI-和VIP免疫反应性神经成分与外侧纵神经索相关。还检查了体外培养29天的原头蚴，并记录了神经解剖学变化。蠕虫体内纵神经索及其交叉连接的发育更明显，并且在主要外侧神经索的后端可见一组神经细胞发育。
