Brennan G P, Halton D W, Maule A G, Shaw C
Comparative Neuroendocrinology Research Group, School of Biology and Biochemistry, Queen's University, Belfast, Northern Ireland, UK.
Parasitol Res. 1993;79(5):409-15. doi: 10.1007/BF00931831.
An electron immunogold-labeling technique was used in conjunction with a post-embedding procedure to demonstrate for the first time the ultrastructural distribution of the parasitic platyhelminth neuropeptide, neuropeptide F (NPF), in the nervous system of the cestode Moniezia expansa. Two axon types, distinguished by their populations of different-sized electron-dense vesicles, were identified. Immunogold labeling demonstrated an apparent homogeneity of PP, FMRFamide and NPF (M. expansa) antigenic sites throughout the larger dense-cored vesicles within the central nervous system. Triple labeling clearly demonstrated the co-localisation of immunoreactivities (IR) for NPF, PP and FMRFamide within the same dense-cored vesicles. The presence of NPF-IR within the vesicles occupying the perikaryon of the neuronal cell body indicated that the peptides had undergone post-translational C-terminal amidation prior to entering the axon. Antigen pre-absorption experiments using NPF prevented labeling with either PP or FMRFamide antisera, and the failure of these antisera to block NPF-IR supports the view that some, if not all, of the PP/FMRFamide-IR is due to NPF-like peptides.
采用电子免疫金标记技术并结合包埋后程序，首次在扩展莫尼茨绦虫的神经系统中展示了寄生扁形虫神经肽神经肽F（NPF）的超微结构分布。通过不同大小电子致密囊泡的群体区分出两种轴突类型。免疫金标记显示，在中枢神经系统内较大的致密核心囊泡中，PP、FMRF酰胺和NPF（扩展莫尼茨绦虫）抗原位点明显均匀分布。三重标记清楚地表明，NPF、PP和FMRF酰胺的免疫反应性（IR）在同一致密核心囊泡中共定位。占据神经元细胞体周核的囊泡内存在NPF-IR，表明这些肽在进入轴突之前经历了翻译后C末端酰胺化。使用NPF的抗原预吸收实验阻止了用PP或FMRF酰胺抗血清进行的标记，并且这些抗血清未能阻断NPF-IR，这支持了这样一种观点，即部分（如果不是全部）PP/FMRF酰胺-IR是由于类似NPF的肽引起的。
