Edgar B A, Kiehle C P, Schubiger G
Cell. 1986 Jan 31;44(2):365-72. doi: 10.1016/0092-8674(86)90771-3.
We have studied the role of the nucleo-cytoplasmic ratio in the early development of Drosophila, using mutants and experimental manipulations that alter nuclear density. Haploid embryos produced by either maternal or paternal effect mutations compensate for haploidy by an extra nuclear division during the syncytial blastoderm stage. Decreasing the nucleo-cytoplasmic ratio in wild-type embryos by ligation can cause a similar extra blastoderm division. Conversely, increasing this ratio can cause the omission of a blastoderm division. The duration of mitotic cycles is affected by the nucleo-cytoplasmic ratio four cycles before the terminal blastoderm division. Transcription patterns in haploid embryos indicate that transcriptional activation is not directly controlled by the nucleo-cytoplasmic ratio, but may be an effect of the lengthening of interphase periods.
我们利用改变核密度的突变体和实验操作，研究了核质比在果蝇早期发育中的作用。由母本或父本效应突变产生的单倍体胚胎，通过在合胞体胚盘阶段进行额外的核分裂来补偿单倍性。通过结扎降低野生型胚胎中的核质比，可导致类似的额外胚盘分裂。相反，增加这个比例会导致胚盘分裂缺失。有丝分裂周期的持续时间在终末胚盘分裂前四个周期受到核质比的影响。单倍体胚胎中的转录模式表明，转录激活并非直接受核质比控制，而可能是间期延长的结果。
