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在固体培养基上短期培养后,通过基质辅助激光解吸电离飞行时间质谱法快速鉴定念珠菌属。

Rapid identification of Candida sp. by MALDI-TOF mass spectrometry subsequent to short-term incubation on a solid medium.

作者信息

Bellanger Anne-Pauline, Gbaguidi-Haore Houssein, Liapis Eleni, Scherer Emeline, Millon Laurence

机构信息

Parasitology Mycology Department, Jean Minjoz University Hospital, Besancon, France.

Chrono-Environment UMR/CNRS 6249, University of Bourgogne Franche-Comté, Besançon, France.

出版信息

APMIS. 2019 Apr;127(4):217-221. doi: 10.1111/apm.12936.

Abstract

Rapid identification of Candida species is important for appropriate antifungal therapy of fungemia. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system is a useful tool to identify bacteria and yeasts. In this study, we evaluated the feasibility of identifying yeasts after a short-term incubation on a solid medium. We tested 24 strains of eight Candida species. Blood culture bottles were spiked with a calibrated suspension of each Candida strain. Three different culture media, two types of blood culture bottles and three different incubation time points were tested. A multivariable random-effects logistic regression analysis was performed for determining factors independently associated with a successful MALDI-TOF MS identification. One-hundred and thirty-one out of 432 MALDI-TOF MS analyses (30%) exhibited a score ≥ 1.7. The performance of the technique varied across Candida species. Factors associated with a successful identification were the use of a chromogenic Candida medium and the time points 4 and 5 h. Using the factors 'chromogenic Candida medium' and time point 5 h the global performance of identification reached 60% and a mean MALDI-TOF score of 1.78. Identifying yeasts after a short-term incubation on a solid medium seems possible, especially when using a chromogenic Candida medium and respecting at least 5 h of incubation. This assay was a first step and needs to be completed using more strains, various chromogenic Candida medium and maybe also testing a longer culture time such as 6 h.

摘要

快速鉴定念珠菌属对于真菌血症的适当抗真菌治疗很重要。基质辅助激光解吸/电离飞行时间质谱(MALDI-TOF MS)系统是鉴定细菌和酵母的有用工具。在本研究中,我们评估了在固体培养基上短期培养后鉴定酵母的可行性。我们测试了8种念珠菌属的24株菌株。将每种念珠菌菌株的校准悬浮液加入血培养瓶中。测试了三种不同的培养基、两种类型的血培养瓶和三个不同的培养时间点。进行多变量随机效应逻辑回归分析以确定与成功的MALDI-TOF MS鉴定独立相关的因素。432次MALDI-TOF MS分析中有131次(30%)的得分≥1.7。该技术的性能因念珠菌属种类而异。与成功鉴定相关的因素是使用显色念珠菌培养基以及4小时和5小时的时间点。使用“显色念珠菌培养基”和5小时时间点这两个因素,鉴定的总体性能达到60%,平均MALDI-TOF得分为1.78。在固体培养基上短期培养后鉴定酵母似乎是可行的,特别是当使用显色念珠菌培养基并至少培养5小时时。该试验是第一步,需要使用更多菌株以及各种显色念珠菌培养基并可能测试更长的培养时间(如6小时)来完成。

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