直接 MALDI-TOF 质谱法检测血培养肉汤用于快速鉴定引起血流感染的念珠菌属种：两个大型微生物实验室的观察性研究。
Direct maldi-tof mass spectrometry assay of blood culture broths for rapid identification of Candida species causing bloodstream infections: an observational study in two large microbiology laboratories.
机构信息
Istituto di Microbiologia,a Istituto di Clinica delle Malattie Infettive, Rome, Italy.
出版信息
J Clin Microbiol. 2012 Jan;50(1):176-9. doi: 10.1128/JCM.05742-11. Epub 2011 Nov 16.
Abstract
We evaluated the reliability of the Bruker Daltonik's MALDI Biotyper system in species-level identification of yeasts directly from blood culture bottles. Identification results were concordant with those of the conventional culture-based method for 95.9% of Candida albicans (187/195) and 86.5% of non-albicans Candida species (128/148). Results were available in 30 min (median), suggesting that this approach is a reliable, time-saving tool for routine identification of Candida species causing bloodstream infection.
摘要
我们评估了 Bruker Daltonik 的 MALDI Biotyper 系统在直接从血培养瓶中鉴定酵母菌种水平方面的可靠性。鉴定结果与传统基于培养的方法一致，对于 95.9%的白色念珠菌（187/195）和 86.5%的非白色念珠菌属酵母菌（128/148）。结果在 30 分钟（中位数）内可用，表明这种方法是一种可靠的、节省时间的用于常规鉴定引起血流感染的念珠菌的工具。
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