School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, 710055 Shaanxi, Xi'an, China; Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, China; Key Laboratory of Environmental Engineering, Shaanxi Province, China; Key Laboratory of Membrane Separation of Shaanxi Province, Xi'an University of Architecture and Technology, Xi'an 710055, China.
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, 710055 Shaanxi, Xi'an, China; Key Laboratory of Northwest Water Resources, Environment and Ecology, MOE, China; Key Laboratory of Environmental Engineering, Shaanxi Province, China; Key Laboratory of Membrane Separation of Shaanxi Province, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Waste Manag. 2019 Feb 15;85:361-373. doi: 10.1016/j.wasman.2018.12.041. Epub 2019 Jan 11.
The objective of this study is to explore how to stimulate soil indigenous bacteria for the degradation of long-chain crude oil by adding fermented food waste supernatant (FS). Four concentrations of FS (0 mL, 0.1 mL, 1 mL, and 3 mL) were added to two oil-contaminated soils S1 and S2 for 30 days of bioremediation experiments. The results showed that the biodegradation of long-chain alkanes (C - C) could reach up to 1756 mg/kg (49.3%, S1) and 3937 mg/kg (43.9%, S2), which were 3.1 and 3.2 times that of the non-nutrient system. In addition, the logarithmic growth rate of the indigenous hydrocarbon degraders (IHD) reached 41.5%. The long-chain crude oil can be rapidly degraded by indigenous bacteria with FS added in a short time. The glucose and acetic acid accelerated the consumption of ammonia nitrogen (NH-N) in the prophase of bioremediation and the molar ratio of consumed carbon (contained in glucose and acetic acid) to consumed NH-N (C/N) was high by adding FS. Thus, the IHD can multiply rapidly. The analysis of microbial diversity revealed that the IHD (genera Acinetobacter and Aquabacterium) became the dominant bacteria. Long-chain alkanes became the main carbon sources for IHD after 14 days in soil S1 and 16 days in soil S2. Thus, the rapid biodegradation of long-chain crude oil was achieved. The genus Aquabacterium which was uncultivable on crude oil medium became the dominant bacteria. This study provides an environment-friendly and sustainable remediation technology for bioremediation of oil-contaminated soils.
本研究旨在通过添加发酵食品废物上清液（FS）来探索如何刺激土壤本土细菌降解长链原油。将四种浓度的 FS（0mL、0.1mL、1mL 和 3mL）添加到两种受油污染的土壤 S1 和 S2 中进行 30 天的生物修复实验。结果表明，长链烷烃（C-C）的生物降解率可达 1756mg/kg（49.3%，S1）和 3937mg/kg（43.9%，S2），分别是非营养体系的 3.1 倍和 3.2 倍。此外，土著烃降解菌（IHD）的对数增长率达到 41.5%。在短时间内，添加 FS 可使本土细菌迅速降解长链原油。在生物修复的前期，葡萄糖和乙酸加速了氨氮（NH-N）的消耗，添加 FS 使消耗的碳（葡萄糖和乙酸中所含）与消耗的 NH-N 的摩尔比（C/N）较高。因此，IHD 可以迅速繁殖。微生物多样性分析表明，IHD（不动杆菌属和Aquabacterium 属）成为优势细菌。在 S1 土壤中 14 天后和 S2 土壤中 16 天后，长链烷烃成为 IHD 的主要碳源。因此，实现了长链原油的快速生物降解。不能在原油培养基上生长的Aquabacterium 属成为优势细菌。本研究为受油污染土壤的生物修复提供了一种环保且可持续的修复技术。
