Mukjang Nilita, Chitov Thararat, Mhuantong Wuttichai, Champreda Verawat, Pathom-Aree Wasu, Sattayawat Pachara, Bovonsombut Sakunnee
Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
Life (Basel). 2022 Oct 27;12(11):1712. doi: 10.3390/life12111712.
In this study, we aim to investigate the efficiency of crude oil bioremediation through composting and culture-assisted composting. First, forty-eight bacteria were isolated from a crude oil-contaminated soil, and the isolate with the highest crude oil degradation activity, identified as , was selected. The bioremediation was then investigated and compared between crude oil-contaminated soil (S), the contaminated soil composted with fruit-based waste (SW), and the contaminated soil composted with the same waste with the addition of the selected bacterium (SWB). Both compost-based methods showed high efficiencies of crude oil bioremediation (78.1% and 83.84% for SW and SWB, respectively). However, only a slight difference between the treatments without and with the addition of was observed. To make a clear understanding of this point, bacterial communities throughout the 4-week bioremediation period were analyzed. It was found that the community dynamics between both composted treatments were similar, which corresponds with their similar bioremediation efficiencies. Interestingly, disappeared from the system after one week, which suggests that this genus was not the key degrader or only involved in the early stage of the process. Altogether, our results elaborate that fruit-based composting is an effective approach for crude oil bioremediation.
在本研究中，我们旨在研究通过堆肥和培养辅助堆肥进行原油生物修复的效率。首先，从受原油污染的土壤中分离出48种细菌，并选择了原油降解活性最高的分离株，鉴定为 。然后，对受原油污染的土壤（S）、与水果基废物堆肥的污染土壤（SW）以及添加所选细菌与相同废物堆肥的污染土壤（SWB）之间的生物修复进行了研究和比较。两种基于堆肥的方法都显示出较高的原油生物修复效率（SW和SWB分别为78.1%和83.84%）。然而，在不添加和添加 的处理之间仅观察到细微差异。为了清楚地理解这一点，分析了整个4周生物修复期间的细菌群落。结果发现，两种堆肥处理之间的群落动态相似，这与它们相似的生物修复效率相对应。有趣的是， 在一周后从系统中消失，这表明该属不是关键降解菌，或仅参与该过程的早期阶段。总之，我们的结果表明，水果基堆肥是原油生物修复的有效方法。
