Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto, 860-0082, Japan.
Division of Applied Life Science, Graduate School of Engineering, Sojo University, 4-22-1 Ikeda, Kumamoto, 860-0082, Japan.
Biochem Biophys Res Commun. 2019 Apr 9;511(3):504-509. doi: 10.1016/j.bbrc.2019.02.116. Epub 2019 Feb 23.
The anti-metastatic effects of cationic liposomes (CL) composed of 87 mol% dimyristoylphosphatidylcholine (DMPC), 8 mol% O,O'-ditetradecanoyl-N-(α-trimethylammonioacetyl) diethanolamine chloride (2CECl) and 5 mol% polyoxyethylene(21) dodecyl ether (C(EO)) was investigated for human pancreatic cancer (BxPC-3) cells. The inhibitory effect of CL on the migration of BxPC-3 cells was observed based on a wound scratch assay. CL suppressed pseudopodium formation of BxPC-3 cells. The anti-invasive effect of CL against BxPC-3 cells was observed via a Matrigel invasion assay. The anti-invasive effect of CL for BxPC-3 cells was found to occur through the inhibition of MMP2, MMP9, and MMP14. Overall, the results of this study revealed for the first time, the therapeutic effects and anti-metastasis activity of CL in xenograft mouse models for peritoneal metastasis of human pancreatic cancer.
研究了由 87mol%二肉豆蔻酰磷脂酰胆碱（DMPC）、8mol%O,O'-二肉豆蔻酰-N-（α-三甲基铵基乙酰）二乙醇胺氯化物（2CECl）和 5mol%聚氧乙烯（21）十二烷基醚（C（EO））组成的阳离子脂质体（CL）对人胰腺癌细胞（BxPC-3）的抗转移作用。通过划痕实验观察 CL 对 BxPC-3 细胞迁移的抑制作用。CL 抑制 BxPC-3 细胞伪足的形成。通过 Matrigel 侵袭实验观察 CL 对 BxPC-3 细胞的抗侵袭作用。发现 CL 对 BxPC-3 细胞的抗侵袭作用是通过抑制 MMP2、MMP9 和 MMP14 发生的。总的来说，这项研究首次揭示了 CL 在人胰腺癌细胞腹膜转移异种移植小鼠模型中的治疗效果和抗转移活性。
