淋巴细胞的分化：B细胞特异性血清抑制因子的证据
Differentiation of lymphoid cells: evidence for a B-cell specific serum suppressor.
作者信息
Kern M
出版信息
Immunology. 1978 Jul;35(1):57-61.
The induction of immunoglobulin production by rabbit spleen cells is markedly inhibited by the presence of normal rabbit serum during cell culture. A similar inhibition is observed when spleen cell populations in which T cells have been inactivated are temporarily incubated with normal rabbit serum before being reconstituted with T cells by adding thymocytes. In contrast, no inhibition was observed upon temporary incubation of thymocytes with normal serum prior to addition of T cell-inactivated spleen cell populations. Removal of adherent cells did not affect the induction of immunoglobulin production or its inhibition by normal serum. Lipopolysaccharide-enhanced immunoglobin production was also inhibited by normal serum, thereby providing additional confidence that bone-marrow derived (B) cells are the target of the normal serum inhibitor.
在细胞培养过程中，正常兔血清的存在会显著抑制兔脾细胞免疫球蛋白的产生。当已使T细胞失活的脾细胞群体在通过添加胸腺细胞重新加入T细胞之前，先用正常兔血清进行短暂孵育时，也会观察到类似的抑制作用。相反，在添加T细胞失活的脾细胞群体之前，将胸腺细胞与正常血清进行短暂孵育则未观察到抑制作用。去除贴壁细胞并不影响免疫球蛋白产生的诱导或正常血清对其的抑制作用。脂多糖增强的免疫球蛋白产生也受到正常血清的抑制，从而进一步证明骨髓来源的（B）细胞是正常血清抑制剂的作用靶点。
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