Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200, Afyonkarahisar, Turkey.
Bayat Vocational School, Afyon Kocatepe University, Afyonkarahisar, Turkey.
Environ Sci Pollut Res Int. 2019 Apr;26(11):11427-11437. doi: 10.1007/s11356-019-04585-5. Epub 2019 Feb 25.
It is claimed that oxidative stress has a prominent role in the mechanism of toxic effects formed by glyphosate-based herbicide (GBH) in living systems. A strong thiol compound, N-acetylcysteine (NAC), has antioxidative and cytoprotective properties. The objective in this subchronic toxicity study was to identify the prophylactic effect of NAC over histopathological changes and oxidative stress induced by GBH in blood, renal, liver, cardiac, and brain tissues. A sum of 28 male Wistar rats were divided into four equal groups, each containing 7 rats. During the study, group I (control group) was supplied with normal rodent bait and tap water ad libitum. The applied agents were 160 mg/kg NAC to group II, 375 mg/kg as equivalent to 1/10 of lethal dose 50% (LD50) of GBH to group III, and 160 mg/kg of NAC and 375 mg/kg of GBH together once per day as oral gavage to group IV for 8 weeks. While GBH decreased the levels of GSH in blood, liver, kidney, and brain tissues, it considerably increased malondialdehyde levels. On the contrary, these parameters happened to improve in the group supplied with NAC. Besides, it was seen that NAC was observed to improve the histopathologic changes in rat tissues induced by GBH. It was concluded that NAC protects oxidative stress and tissue damage induced by GBH in blood and tissue and this prophylactic effect could be attributed to its antioxidant and free radical sweeper character.
据称，氧化应激在草甘膦基除草剂（GBH）在活系统中形成的毒性作用机制中具有突出作用。一种强硫醇化合物，N-乙酰半胱氨酸（NAC），具有抗氧化和细胞保护特性。本亚慢性毒性研究的目的是确定 NAC 对 GBH 在血液、肾脏、肝脏、心脏和脑组织中诱导的组织病理学变化和氧化应激的预防作用。总共 28 只雄性 Wistar 大鼠被分为四组，每组 7 只。在研究期间，第 I 组（对照组）给予正常啮齿动物诱饵和自来水自由摄取。第 II 组给予 160mg/kg 的 NAC，第 III 组给予相当于草甘膦半数致死剂量 50%（LD50）的 1/10 的 375mg/kg，第 IV 组每天口服给予 160mg/kg 的 NAC 和 375mg/kg 的 GBH，持续 8 周。虽然 GBH 降低了血液、肝脏、肾脏和脑组织中的 GSH 水平，但它大大增加了丙二醛水平。相反，在给予 NAC 的组中，这些参数有所改善。此外，还观察到 NAC 可改善由 GBH 引起的大鼠组织的组织病理学变化。研究结论为，NAC 可预防 GBH 引起的血液和组织中的氧化应激和组织损伤，这种预防作用可能归因于其抗氧化和自由基清除特性。
