衰老造血干祖细胞中与错配修复相关的差异表达基因的RNA测序分析
RNA-Seq analysis of differentially expressed genes relevant to mismatch repair in aging hematopoietic stem-progenitor cells.
作者信息
Lian Xiaolan, Dong Yongpin, Zhao Mingyi, Liang Yajie, Jiang Weiwei, Li Wenfang, Zhang Lina
机构信息
Department of Biochemistry, Institute of Basic Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Fujian Provincial Key Laboratory on Hematology, Fujian Institute of Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
出版信息
J Cell Biochem. 2019 Jul;120(7):11401-11408. doi: 10.1002/jcb.28417. Epub 2019 Feb 25.
We used RNA-sequencing (RNA-Seq) technology and an old hematopoietic stem and progenitor cells (HSPCs) model in vitro to analyze differential expressions of mismatch repair (MMR)-related genes in aged HSPCs, so as to explore the mechanism of DNA MMR injury in hematopoietic stem cells (HSC) aging. In this study, by combining RNA-seq data and National Center for Biotechnology Information database, we focus on six widely reported MMR genes MSH2, MSH3, MSH6, MLH1, PMS1, PMS2, and five MMR genes with closer ties to HSC aging Pcna, Exo1, Rpa1, Rpa2, and Rpa3 according to the genes functional classification and the related signaling pathway. It is concluded that MMR is closely related to HSC aging. This study provides experimental evidence for future researching MMR in HSC aging.
我们利用RNA测序（RNA-Seq）技术和体外旧的造血干细胞和祖细胞（HSPCs）模型，分析老年HSPCs中错配修复（MMR）相关基因的差异表达，以探讨造血干细胞（HSC）衰老过程中DNA错配修复损伤的机制。在本研究中，通过整合RNA测序数据和美国国立生物技术信息中心数据库，根据基因功能分类和相关信号通路，我们重点关注六个广泛报道的MMR基因MSH2、MSH3、MSH6、MLH1、PMS1、PMS2，以及五个与HSC衰老关系更密切的MMR基因Pcna、Exo1、Rpa1、Rpa2和Rpa3。得出MMR与HSC衰老密切相关的结论。本研究为未来研究HSC衰老中的MMR提供了实验证据。
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