Aboushousha Tarek, Lashen Rana, Abdelnaser Khadega, Helal Noha, Moussa Mona, Omran Zeinab, Eldahshan Samir, El Ganzoury Hossam
Department of Pathology, Theodor Bilharz Research Institute, Cairo, Egypt. Email:
Asian Pac J Cancer Prev. 2019 Feb 26;20(2):615-620. doi: 10.31557/APJCP.2019.20.2.615.
Background: Prostate cancer (PCa) is a common health problem in elderly. RAGE (Receptor for advanced glycation end products) is overexpressed in multiple human cancers. SOX2 (Sex-determining region Y box 2) also functions as an oncoprotein and promotes cancer progression but the mechanisms involved remain largely unknown. Aim: The current study investigated the expression patterns of RAGE and SOX2 in benign and malignant prostate samples in correlation with the histopathological findings in order to evaluate their role as prognostic markers or therapeutic targets. Methods: Immunohistochemical staining for RAGE and SOX2 antibodies was applied on 87 prostatic biopsies [16 of prostatitis, 20 of benign prostatic hyperplasia (BPH) and 51 of PCa]. Results: Expression of RAGE and SOX2 (percentage of positive cells) was significantly higher in PCa lesions compared with prostatitis (p<0.01) and BPH (p<0.0001) and was also significantly higher in prostatitis compared with BPH lesions (p<0.01). Also, percentage of positive RAGE and SOX2 cells showed a significant stepwise increase from Gleason Grade 3 to Grade 5 and were significantly higher in high Gleason Scores (≥8) compared to lower Scores (≤7) with statistical significance (p=0.001). Conclusion: RAGE and SOX2 were up-regulated in prostate cancer lesions, mainly in advanced grades, suggesting an active role of both antigens in the development and progression of prostate cancer and expecting the possibility of their use as therapeutic targets.
前列腺癌（PCa）是老年人常见的健康问题。晚期糖基化终产物受体（RAGE）在多种人类癌症中过度表达。性别决定区Y框蛋白2（SOX2）也作为一种癌蛋白发挥作用并促进癌症进展，但其涉及的机制在很大程度上仍不清楚。目的：本研究调查RAGE和SOX2在良性和恶性前列腺样本中的表达模式，并与组织病理学结果相关联，以评估它们作为预后标志物或治疗靶点的作用。方法：对87例前列腺活检组织[16例前列腺炎、20例良性前列腺增生（BPH）和51例PCa]进行RAGE和SOX2抗体的免疫组织化学染色。结果：与前列腺炎（p<0.01）和BPH（p<0.0001）相比，PCa病变中RAGE和SOX2的表达（阳性细胞百分比）显著更高，与BPH病变相比，前列腺炎中的表达也显著更高（p<0.01）。此外，RAGE和SOX2阳性细胞百分比从Gleason分级3级到5级呈显著逐步增加，与较低评分（≤7）相比，高Gleason评分（≥8）中的表达显著更高，具有统计学意义（p=0.001）。结论：RAGE和SOX2在前列腺癌病变中上调，主要在高级别中，表明这两种抗原在前列腺癌的发生和进展中发挥积极作用，并有望将其用作治疗靶点。
