1Department of Nutritional Sciences,University of Connecticut,Storrs,CT 06269,USA.
Br J Nutr. 2019 Apr;121(7):748-755. doi: 10.1017/S0007114519000126. Epub 2019 Feb 26.
Treatment of liver fibrosis is very limited as there is currently no effective anti-fibrotic therapy. Spirulina platensis (SP) is a blue-green alga that is widely supplemented in healthy foods. The objective of this study was to determine whether SP supplementation can prevent obesity-induced liver fibrosis in vivo. Male C57BL/6J mice were randomly assigned to a low-fat or a high-fat (HF)/high-sucrose/high-cholesterol diet or an HF diet supplemented with 2·5 % SP (w/w) (HF/SP) for 16 or 20 weeks. There were no significant differences in body weight, activity, energy expenditure, serum lipids or glucose tolerance between mice on HF and HF/SP diets. However, plasma alanine aminotransferase level was significantly reduced by SP at 16 weeks. Expression of fibrotic markers and trichrome stains showed no differences between HF and HF/SP. Splenocytes isolated from HF/SP fed mice had lower inflammatory gene expression and cytokine secretion compared with splenocytes from HF-fed mice. SP supplementation did not attenuate HF-induced liver fibrosis. However, the expression and secretion of inflammatory genes in splenocytes were significantly reduced by SP supplementation, demonstrating the anti-inflammatory effects of SP in vivo. Although SP did not show appreciable effect on the prevention of liver fibrosis in this mouse model, it may be beneficial for other inflammatory conditions.
目前尚无有效的抗纤维化疗法，因此肝纤维化的治疗非常有限。螺旋藻是一种广泛添加于健康食品中的蓝绿藻。本研究旨在确定螺旋藻补充剂是否能预防肥胖诱导的肝纤维化。雄性 C57BL/6J 小鼠被随机分为低脂或高脂肪（HF）/高蔗糖/高胆固醇饮食组，或 HF 饮食添加 2.5%螺旋藻（w/w）（HF/SP）组，喂养 16 或 20 周。HF 和 HF/SP 饮食组的小鼠体重、活动量、能量消耗、血脂或葡萄糖耐量均无显著差异。然而，16 周时 SP 可显著降低血浆丙氨酸转氨酶水平。纤维化标志物和三染色显示 HF 和 HF/SP 之间无差异。与 HF 喂养的小鼠相比，来自 HF/SP 喂养的小鼠的脾细胞的炎症基因表达和细胞因子分泌较低。SP 补充并未减轻 HF 诱导的肝纤维化。然而，SP 补充可显著降低脾细胞中炎症基因的表达和分泌，表明 SP 在体内具有抗炎作用。尽管在该小鼠模型中，螺旋藻对预防肝纤维化没有明显作用，但它可能对其他炎症状况有益。
