College of Food Science and Engineering, Qilu University of Technology, No.3501, Daxue Road, Jinan 250353, China.
Department of Nutrition and Food Hygiene, School of Public Health, Shandong University, No.44 Wenhuaxi Road, Jinan 250012, China.
Mar Drugs. 2020 Mar 4;18(3):148. doi: 10.3390/md18030148.
High-fat and high-sucrose diet (HFHSD)-induced obesity leads to oxidative stress and chronic inflammatory status. However, little is known about the beneficial effects of total lipids extracted from . Hence, in the present study, lipids were extracted with chloroform/methanol (SLC) or ethanol (SLE) and then their effects on oxidative stress and inflammation in the mice fed a HFHSD were investigated. The results show that the major lipid classes and fatty acid profiles of SLC and SLE were almost similar, but the gamma-linolenic acid (GLA) and carotenoid contents in SLE was a little higher than that in SLC. Dietary 4% SLC or SLE for 12 weeks effectively decreased the hepatic lipid hydroperoxide levels as well as increased the activities and mRNA levels of antioxidant enzymes in the mice fed a HFHSD. In addition, supplementation with SLC and SLE also markedly decreased the levels of serum pro-inflammatory cytokines and the mRNA expression of pro-inflammatory cytokines in the liver and epididymal white adipose tissue of mice fed a HFHSD, and the effects of SLC and SLE were comparable. These findings confirm for the first time that dietary lipids could alleviate HFHSD-induced oxidative stress and inflammation.
高脂肪高糖饮食（HFHSD）诱导的肥胖会导致氧化应激和慢性炎症状态。然而，人们对 中提取的总脂质的有益作用知之甚少。因此，在本研究中，用氯仿/甲醇（SLC）或乙醇（SLE）提取 脂质，然后研究它们对 HFHSD 喂养小鼠氧化应激和炎症的影响。结果表明，SLC 和 SLE 的主要脂质类和脂肪酸谱几乎相似，但 SLE 中的γ-亚麻酸（GLA）和类胡萝卜素含量略高于 SLC。12 周的饮食 4% SLC 或 SLE 可有效降低 HFHSD 喂养小鼠肝脏脂质氢过氧化物水平，并增加抗氧化酶的活性和 mRNA 水平。此外，SLC 和 SLE 的补充还显著降低了 HFHSD 喂养小鼠血清促炎细胞因子水平和肝脏及附睾白色脂肪组织中促炎细胞因子的 mRNA 表达，且 SLC 和 SLE 的作用相当。这些发现首次证实，饮食中 脂质可减轻 HFHSD 诱导的氧化应激和炎症。
