College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China.
Center for Viticulture and Enology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China.
Plant Mol Biol. 2019 May;100(1-2):111-132. doi: 10.1007/s11103-019-00847-5. Epub 2019 Feb 26.
The whole promoter regions of SUTs in Vitis were firstly isolated. SUTs are involved in the adaptation to biotic and abiotic stresses. The vulnerability of Vitis vinifera to abiotic and biotic stresses limits its yields. In contrast, Vitis amurensis displays resistance to environmental stresses, such as microbial pathogens, low temperatures, and drought. Sucrose transporters (SUTs) are important regulators for plant growth and stress tolerance; however, the role that SUTs play in stress resistance in V. amurensis is not known. Using V. amurensis Ruper. 'Zuoshan-1' and V. vinifera 'Chardonnay', we found that SUC27 was highly expressed in several vegetative organs of Zuoshan-1, SUC12 was weakly expressed or absent in most organs in both the species, and the distribution of SUC11 in source and sink organs was highest in Zuoshan-1. A search for cis-regulatory elements in the promoter sequences of SUTs revealed that they were regulated by light, environmental stresses, physiological correlation, and hormones. The SUTs in Zuoshan-1 mostly show a higher and rapid response than in Chardonnay under the induction by Plasmopara viticola infection, cold, water deficit, and dark conditions. The induction of SUTs was associated with the upregulation of key genes involved in sucrose metabolism and the biosynthesis of plant hormones. These results indicate that stress resistance in Zuoshan-1 is governed by the differential distribution and induction of SUTs by various stimuli, and the subsequent promotion of sucrose metabolism and hormone synthesis.
首次从葡萄中分离出 SUT 全启动子区域。SUT 参与生物和非生物胁迫的适应。葡萄对生物和非生物胁迫的脆弱性限制了其产量。相比之下，山葡萄对环境胁迫如微生物病原体、低温和干旱具有抗性。蔗糖转运蛋白（SUTs）是植物生长和胁迫耐受的重要调节剂；然而，SUTs 在山葡萄中的胁迫抗性中的作用尚不清楚。使用山葡萄 Ruper. 'Zuoshan-1' 和酿酒葡萄 '霞多丽'，我们发现 SUC27 在 Zuoshan-1 的几个营养器官中高度表达，SUC12 在两个物种的大多数器官中表达较弱或不存在，而 SUC11 在源和汇器官中的分布在 Zuoshan-1 中最高。对 SUT 启动子序列中的顺式调控元件进行搜索发现，它们受光、环境胁迫、生理相关性和激素的调控。在 Plasmopara viticola 感染、寒冷、水分亏缺和黑暗条件下，山葡萄中的 SUT 大多表现出比霞多丽更高和更快的响应。SUT 的诱导与蔗糖代谢和植物激素生物合成中关键基因的上调有关。这些结果表明，山葡萄的抗胁迫能力是由各种刺激下 SUT 的差异分布和诱导以及随后促进蔗糖代谢和激素合成所决定的。
