a Department of Horticulture, Sunchon National University, Suncheon 57922, Republic of Korea.
b Department of Genetics and Plant Breeding, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh.
Genome. 2019 Apr;62(4):253-266. doi: 10.1139/gen-2018-0173. Epub 2019 Feb 26.
Broccoli ( var. L.) is a highly nutritious vegetable that typically forms pure green or purple florets. However, green broccoli florets sometimes accumulate slight purplish pigmentation in response environmental factors, decreasing their market value. In the present study, we aimed to develop molecular markers to distinguish broccoli genotypes as pure green or purplish floret color at the early seedling stage. Anthocyanins are known to be involved in the purple pigmentation in plants. The purplish broccoli lines were shown to accumulate purple pigmentation in the hypocotyls of very young seedlings; therefore, the expression profiles of the structural and regulatory genes of anthocyanin biosynthesis were analyzed in the hypocotyls using qRT-PCR. , , , , , , and were identified as putative candidate genes responsible for the purple hypocotyl color. was much more highly expressed in the purple than green hypocotyls; therefore, it was cloned and sequenced from various broccoli lines, revealing SNP and InDel variations between these genotypes. We tested four SNPs (G > A; A > T; G > C; T > G) in the first three exons and a 14-bp InDel (ATATTTATATATAT) in the promoter in 51 broccoli genotypes, and we found these genetic variations could distinguish the green lines, purple lines, and F hybrids. These novel molecular markers could be useful in broccoli breeding programs to develop a true green or purple broccoli cultivar.
西兰花（var. L.）是一种营养丰富的蔬菜，通常形成纯绿色或紫色花球。然而，绿色西兰花花球有时会因环境因素积累轻微的紫色色素，降低其市场价值。在本研究中，我们旨在开发分子标记物，以区分西兰花基因型为纯绿色或紫色花球颜色在早期幼苗阶段。花色素苷是植物中紫色色素的已知参与物质。研究表明，紫色西兰花品系在非常幼小的幼苗下胚轴中积累紫色色素；因此，使用 qRT-PCR 分析了下胚轴中花色素苷生物合成的结构和调节基因的表达谱。鉴定出 、 、 、 、 和 为负责紫色下胚轴颜色的假定候选基因。在紫色下胚轴中 表达量明显高于绿色下胚轴；因此，它从各种西兰花品系中克隆并测序，揭示了这些基因型之间的 SNP 和 InDel 变异。我们在 51 个西兰花基因型中测试了第一三个外显子中的四个 SNP（G > A；A > T；G > C；T > G）和 启动子中的 14-bp InDel（ATATTTATATATAT），发现这些遗传变异可以区分绿色品系、紫色品系和 F 杂种。这些新的分子标记可用于西兰花育种计划，以开发真正的绿色或紫色西兰花品种。
