Map-based cloning and CRISPR/Cas9-based editing uncover as the causal gene for the no-anthocyanin-accumulation phenotype in curly kale ( var. ).

comprises several important vegetable and ornamental crops, including curly kale, ornamental kale, cabbage, broccoli, and others. The accumulation of anthocyanins, important secondary metabolites valuable to human health, in these plants varies widely and is responsible for their pink to dark purple colors. Some curly kale varieties lack anthocyanins, making these plants completely green. The genetic basis of this trait is still unknown. We crossed the curly kale inbred line BK2019 (without anthocyanins) with the cabbage inbred line YL1 (with anthocyanins) and the Chinese kale inbred line TO1000 (with anthocyanins) to generate segregating populations. The no-anthocyanin trait was genetically controlled by a recessive gene, . We generated a linkage map and mapped to a 256-kb interval on C09. We identified one candidate gene, , in the target genomic region; this gene is homologous to , which encodes a dihydroflavonol-4-reductase-like (DFR-like) protein in . In BK2019, a 1-bp insertion was observed in the second exon of and directly produced a stop codon. To verify the candidate gene function, CRISPR/Cas9 gene editing technology was applied to knock out . We generated three mutants, two of which were completely green with no anthocyanins, confirming that corresponds to Different insertion/deletion mutations in exons were found in all six of the other no-anthocyanin kale varieties examined, supporting that independent disruption of resulted in no-anthocyanin varieties of . This study improves the understanding of the regulation mechanism of anthocyanin accumulation in subspecies.