Food Chemistry, Department of Chemistry and Pharmacy, Emil Fischer Center, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Nikolaus-Fiebiger-Straße 10, 91058, Erlangen, Germany.
Food Chemistry, Department of Chemistry and Pharmacy, Emil Fischer Center, Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Nikolaus-Fiebiger-Straße 10, 91058, Erlangen, Germany.
J Pharm Biomed Anal. 2019 May 10;168:124-132. doi: 10.1016/j.jpba.2019.02.022. Epub 2019 Feb 16.
Hop β-bitter acids (lupulones) are health-beneficial components of Humulus lupulus L. showing, for example, antidepressant-like effects in vitro. Despite of the widespread use of hops for medicinal purposes, the concentrations of lupulones in hop-based drugs have not been reported yet. The present study developed, validated, and applied a method with external calibration, which allows for the first time separate quantification of co-, n-, and ad-lupulone in hop-based drugs by UHPLC‒DAD. Concentrations between 'not detectable' and 2.7 mg/mL co-lupulone, 2.2 mg/mL nlupulone, or 0.7 mg/mL ad-lupulone were measured in nine different commercial dietary supplements and phytopharmaceuticals. Only one hop tincture contained sufficient lupulone to possibly exert potential antidepressant effects. Aiming for products with increased lupulone content, the extraction efficiency of different solvents was investigated. Complete extraction of lupulones from raw hops was achieved by organic solvents including methanol and ethanol, whereas aqueous mixtures resulted in low recovery. These results indicate that adapted extraction conditions may result in more effective hops products.
酒花β-酸（蛇麻酮）是蛇麻草 L. 的有益健康成分，具有体外抗抑郁作用。尽管蛇麻草被广泛用于药用，但尚未报道基于蛇麻草的药物中的蛇麻酮浓度。本研究开发、验证并应用了一种外部校准方法，该方法首次允许通过 UHPLC-DAD 分别定量分析基于蛇麻草药物中的 co-、n-和 ad-蛇麻酮。在九种不同的商业膳食补充剂和植物药中，检测到 co-蛇麻酮、nlupulone 和 ad-lupulone 的浓度分别在“未检出”到 2.7mg/mL、2.2mg/mL 和 0.7mg/mL 之间。只有一种蛇麻草酊剂含有足够的蛇麻酮，可能具有潜在的抗抑郁作用。为了提高产品中蛇麻酮的含量，研究了不同溶剂的提取效率。甲醇和乙醇等有机溶剂可从生蛇麻草中完全提取蛇麻酮，而水混合溶剂则导致回收率低。这些结果表明，适应性提取条件可能会产生更有效的蛇麻草产品。
