Aftab Ghazal, Rajaei Seyed Mehdi, Pot Simon A, Faghihi Houman
Department of Clinical Sciences. Faculty of Specialized Veterinary Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Ophthalmology section, Negah Veterinary Centre, Tehran, Iran.
Top Companion Anim Med. 2019 Mar;34:30-32. doi: 10.1053/j.tcam.2018.12.003. Epub 2018 Dec 11.
This study was performed to determine the normal seasonal aerobic and an-aerobic corneoconjunctival bacterial flora in cats. Thirty eyes of 15 clinically normal client-owned Persian cats were evaluated. All cats lived in a similar indoor/outdoor home environment being fed the same diet for the entire year. The cats did not receive any medications and were found to be clinically healthy 1 week prior to each microbial sampling. The cats were not exposed to other cats during the study period. Microbial samples were collected at the same time of day on the first day of the second month of each of the four seasons. During sample collection, a sterile swab was rolled over the corneoconjunctival surface avoiding contact with surrounding skin or hair. Immediately after sample collection, microbiologic aerobic and anaerobic cultures were initiated. Gram-positive bacteria were the most prevalent isolates. The most commonly isolated bacterial organisms across all seasons were Staphylococcus epidermidis (41/95; 43.2%), β-hemolytic streptococcus (18/95; 18.9%), Staphylococcus aureus (17/95; 17.9%), and Escherichia coli (11/95; 11.5%). Twenty-five cultures of a total of 120 (20.8%) were negative. One negative culture was collected in the summer, while 21 cultures were negative in fall and winter. Gram-positive bacteria were the predominant micro-organisms of the normal ocular surface of healthy cats in all seasons in this study. This result is in agreement with previous publications.
本研究旨在确定猫正常季节性需氧和厌氧角膜结膜细菌菌群。对15只临床正常的客户拥有的波斯猫的30只眼睛进行了评估。所有猫都生活在类似的室内/室外家庭环境中，全年喂食相同的饮食。这些猫未接受任何药物治疗，并且在每次微生物采样前1周被发现临床健康。在研究期间，这些猫未接触其他猫。在四个季节中每个季节的第二个月的第一天，在同一天的同一时间采集微生物样本。在样本采集过程中，用无菌拭子在角膜结膜表面滚动，避免接触周围皮肤或毛发。样本采集后立即开始进行微生物需氧和厌氧培养。革兰氏阳性菌是最常见的分离菌。在所有季节中最常分离出的细菌是表皮葡萄球菌（41/95；43.2%）、β-溶血性链球菌（18/95；18.9%）、金黄色葡萄球菌（17/95；17.9%）和大肠杆菌（11/95；11.5%）。在总共120次培养中有25次（20.8%）为阴性。夏季采集到1次阴性培养物，而秋季和冬季有21次培养物为阴性。在本研究中，革兰氏阳性菌是健康猫在所有季节正常眼表的主要微生物。这一结果与先前的出版物一致。
