Büttner Jessica Nina, Schneider Marianne, Csokai Jaqueline, Müller Elisabeth, Eule Johanna Corinna
Small Animal Clinic, Freie Universität Berlin, Berlin, Germany.
LABOKLIN GmbH & Co. KG, Bad Kissingen, Germany.
Vet Ophthalmol. 2019 May;22(3):328-336. doi: 10.1111/vop.12598. Epub 2018 Aug 10.
To describe the bacterial and fungal microbiota of the conjunctiva and factors influencing these microbiota of healthy cats. To evaluate the antimicrobial resistance profile and discuss the use of appropriate antimicrobial drugs.
One hundred and twenty healthy cats PROCEDURES: Conjunctival samples with dry or premoistened swabs were obtained from both eyes and cultured for aerobic and anaerobic bacteria and fungi. In vitro bacterial, susceptibility testing was performed. The effects of age, sex, breed, season, living environment, and sample collection technique on the frequency and composition of microbial isolation were evaluated.
In 49 of 120 cats (40.8%) and 73 of 240 swabs (30.4%), microorganisms were isolated. Of the isolates, 71% (61/86) were Gram-positive bacteria, 26% (22/86) were Gram-negative bacteria, and 3% (3/86) were fungi. Staphylococcus felis (17/86; 19.8%) was the most commonly isolated species. Moraxella osloensis (5/86; 5.8%) was the most frequent Gram-negative species. The season had a statistically significant influence (P = 0.04) on the frequency of isolation. The use of premoistened swabs increased the rate of Gram-positive bacterial detection significantly (P = 0.03). The in vitro susceptibility testing showed high efficacy of chloramphenicol, gentamicin, pradofloxacin, and enrofloxacin.
The isolated microbiota aligns with previous studies performed in other countries, although the microbiota of cats in the present study showed wider bacterial diversity, and more Gram-negative species were isolated. Swab sampling should be performed with premoistened swabs. The topical antimicrobials gentamicin and chloramphenicol are suitable therapeutics for first-line treatment.
描述健康猫结膜的细菌和真菌微生物群以及影响这些微生物群的因素。评估抗菌药物耐药性谱并讨论合适抗菌药物的使用。
120只健康猫
用干燥或预先湿润的拭子从双眼获取结膜样本，进行需氧菌、厌氧菌和真菌培养。进行体外细菌药敏试验。评估年龄、性别、品种、季节、生活环境和样本采集技术对微生物分离频率和组成的影响。
120只猫中的49只（40.8%）以及240个拭子中的73个（30.4%）分离出微生物。分离出的微生物中，71%（61/86）为革兰氏阳性菌，26%（22/86）为革兰氏阴性菌，3%（3/86）为真菌。费氏葡萄球菌（17/86；19.8%）是最常分离出的菌种。奥斯陆莫拉菌（5/86；5.8%）是最常见的革兰氏阴性菌种。季节对分离频率有统计学显著影响（P = 0.04）。使用预先湿润的拭子显著提高了革兰氏阳性菌的检测率（P = 0.03）。体外药敏试验显示氯霉素、庆大霉素、普拉氟沙星和恩诺沙星疗效高。
尽管本研究中猫的微生物群显示出更广泛的细菌多样性且分离出更多革兰氏阴性菌种，但分离出的微生物群与其他国家先前的研究结果一致。应使用预先湿润的拭子进行拭子采样。局部抗菌药物庆大霉素和氯霉素是一线治疗的合适药物。
