Bioorganic Chemistry and Bio-Crystallography laboratory (B2Cl), Faculty of Science and Technology, Free University of Bolzano, Piazza Università 5, 39100, Bolzano, Italy.
Centre for Integrative Biology, University of Trento, via Sommarive n. 9, 38123, Povo, Trento, Italy.
Sci Rep. 2019 Feb 26;9(1):2818. doi: 10.1038/s41598-019-39787-x.
Erwinia amylovora is the etiological agent of fire blight, a devastating disease which is a global threat to commercial apple and pear production. The Erwinia genus includes a wide range of different species belonging to plant pathogens, epiphytes and even opportunistic human pathogens. The aim of the present study is to understand, within the Erwinia genus, the genetic differences between phytopathogenic strains and those strains not reported to be phytopathogenic. The genes related to the hydroxamate siderophores iron uptake have been considered due to their potential druggability. In E. amylovora siderophore-mediated iron acquisition plays a relevant role in the progression of Fire blight. Here we analyzed the taxonomic relations within Erwinia genus and the relevance of the genes related to the siderophore-mediated iron uptake pathway. The results of this study highlight the presence of a well-defined sub-group of Rosaceae infecting species taxonomically and genetically related with a high number of conserved core genes. The analysis of the complete ferrioxamine transport system has led to the identification of two genes exclusively present in the Rosaceae infecting strains.
果胶杆菌是火疫病的病原体，火疫病是一种对商业苹果和梨生产具有全球性威胁的毁灭性疾病。果胶杆菌属包括广泛的不同物种，属于植物病原体、附生菌，甚至是机会性病原体。本研究的目的是在果胶杆菌属内，了解植物病原菌株与未报道为植物病原菌株之间的遗传差异。由于其潜在的可成药性，与羟肟酸类铁载体铁摄取相关的基因已被考虑在内。在果胶杆菌中，铁载体介导的铁摄取在火疫病的发展中起着重要作用。在这里，我们分析了果胶杆菌属内的分类关系以及与铁载体介导的铁摄取途径相关基因的相关性。这项研究的结果强调了存在一个明确的蔷薇科感染种亚群，在分类学和遗传学上与大量保守的核心基因相关。对完整的高铁胺转运系统的分析导致了仅在蔷薇科感染菌株中存在的两个基因的鉴定。
