造血胸腺细胞前体：IV. 前体的富集及异质性证据。
Hematopoietic thymocyte precursors: IV. Enrichment of the precursors and evidence for heterogeneity.
作者信息
Basch R S, Kadish J L, Goldstein G
出版信息
J Exp Med. 1978 Jun 1;147(6):1843-8. doi: 10.1084/jem.147.6.1843.
Abstract
A method has been developed for the enrichment of the hematopoietic precursors of thymocytes from spleen and bone marrow cells. Up to 40-fold enrichments were obtained resulting in preparations in which as few as 10(5) cells produced prompt repopulation of the thymus of an irradiated mouse. Precursor cells from bone marrow appear to contain the enzyme terminal deoxyribonucleotidyl transferase (Tdt), an agent suggested as a potential somatic mutator. This enzyme (Tdt) was not detectable in any spleen cell preparation examined, including one in which a 40-fold enrichment of thymocyte precursors had been produced. This is the first difference reported between the splenic and bone marrow precursors of thymocytes.
摘要
已开发出一种从脾脏和骨髓细胞中富集胸腺细胞造血前体的方法。获得了高达40倍的富集效果，从而得到的细胞制剂中，低至10⁵个细胞就能使受辐照小鼠的胸腺迅速重新填充。来自骨髓的前体细胞似乎含有末端脱氧核苷酸转移酶（Tdt），该酶被认为是一种潜在的体细胞突变诱导剂。在所检测的任何脾脏细胞制剂中均未检测到这种酶（Tdt），包括一种已产生40倍胸腺细胞前体富集的制剂。这是报道的胸腺细胞脾脏前体和骨髓前体之间的首个差异。
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