Hoyeck Myriam P, Hadj-Moussa Hanane, Storey Kenneth B
Institute of Biochemistry, Departments of Biology and Chemistry, Carleton University, Ottawa, Ontario, Canada.
PeerJ. 2019 Feb 20;7:e6515. doi: 10.7717/peerj.6515. eCollection 2019.
When faced with extreme environmental conditions, the milk snail () enters a state of dormancy known as estivation. This is characterized by a strong reduction in metabolic rate to <30% of normal resting rate that is facilitated by various behavioural, physiological, and molecular mechanisms. Herein, we investigated the regulation of microRNA in the induction of estivation. Changes in the expression levels of 75 highly conserved microRNAs were analysed in snail foot muscle, of which 26 were significantly upregulated during estivation compared with controls. These estivation-responsive microRNAs were linked to cell functions that are crucial for long-term survival in a hypometabolic state including anti-apoptosis, cell-cycle arrest, and maintenance of muscle functionality. Several of the microRNA responses by snail foot muscle also characterize hypometabolism in other species and support the existence of a conserved suite of miRNA responses that regulate environmental stress responsive metabolic rate depression across phylogeny.
当面临极端环境条件时，乳螺（）进入一种称为夏眠的休眠状态。其特征是代谢率大幅降低至正常静息率的<30%，这是由各种行为、生理和分子机制促成的。在此，我们研究了微小RNA在夏眠诱导中的调控作用。分析了蜗牛足部肌肉中75种高度保守的微小RNA的表达水平变化，其中26种在夏眠期间与对照相比显著上调。这些对夏眠有反应的微小RNA与细胞功能相关，这些功能对于在低代谢状态下的长期存活至关重要，包括抗凋亡、细胞周期停滞和肌肉功能维持。蜗牛足部肌肉的几种微小RNA反应也表征了其他物种的低代谢，并支持存在一组保守的微小RNA反应，这些反应调节系统发育中对环境应激反应性代谢率降低。
