Huang Songqian, Yoshitake Kazutoshi, Asaduzzaman Md, Kinoshita Shigeharu, Watabe Shugo, Asakawa Shuichi
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
School of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan.
RNA Biol. 2021 Nov;18(11):1702-1715. doi: 10.1080/15476286.2020.1867798. Epub 2021 Jan 7.
Small non-coding RNAs play a pivotal role in gene regulation, repression of transposable element and viral activity in various organisms. Among the various categories of these small non-coding RNAs, microRNAs (miRNAs) guide post-translational gene regulation in cellular development, proliferation, apoptosis, oncogenesis, and differentiation. Here, we performed a genome-wide computational prediction of miRNAs to improve the understanding of miRNA observation and function in molluscs. As an initial step, hundreds of conserved miRNAs were predicted in 35 species of molluscs through genome scanning. Afterwards, the miRNAs' population, isoforms, organization, and function were characterized in detail. Furthermore, the key miRNA biogenesis factors, including AGO2, DGCR8, DICER, DROSHA, TRABP2, RAN, and XPO5, were elucidated based on homologue sequence searching. We also summarized the miRNAs' function in biomineralization, immune and stress response, as well as growth and development in molluscs. Because miRNAs play a vital role in various lifeforms, this study will provide insight into miRNA biogenesis and function in molluscs, as well as other invertebrates.
小非编码RNA在各种生物体的基因调控、转座元件抑制和病毒活性中发挥着关键作用。在这些小非编码RNA的各类中，微小RNA（miRNA）在细胞发育、增殖、凋亡、肿瘤发生和分化过程中指导翻译后基因调控。在此，我们进行了全基因组范围的miRNA计算预测，以增进对软体动物中miRNA的观察和功能的理解。作为第一步，通过基因组扫描在35种软体动物中预测了数百个保守的miRNA。随后，对miRNA的群体、异构体、组织和功能进行了详细表征。此外，基于同源序列搜索阐明了关键的miRNA生物发生因子，包括AGO2、DGCR8、DICER、DROSHA、TRABP2、RAN和XPO5。我们还总结了miRNA在软体动物生物矿化、免疫和应激反应以及生长发育中的功能。由于miRNA在各种生命形式中都起着至关重要的作用，本研究将为软体动物以及其他无脊椎动物中miRNA的生物发生和功能提供深入了解。
