Raff E C, Fuller M T, Kaufman T C, Kemphues K J, Rudolph J E, Raff R A
Cell. 1982 Jan;28(1):33-40. doi: 10.1016/0092-8674(82)90372-5.
Four different tubulins have been identified that are expressed during embryogenesis in Drosophila melanogaster. Two alpha-tubulin subunits (alpha 1 and alpha 2) and one beta-tubulin subunit (beta 1) are expressed throughout embryonic development. A second beta-tubulin subunit (beta 3) is expressed only for a short period in mid-embryonic development. Synthesis of beta 3-tubulin in vitro in a rabbit reticulocyte translation system is directed by RNA extracted from embryos only at the stage when the protein is expressed. Thus we conclude that the mRNA encoding beta 3-tubulin is transcribed only during the brief period of beta 3-tubulin synthesis. The expression of beta 3-tubulin is accompanied by a coordinate transient increase in the level of synthesis of the embryonic alpha-tubulins, thereby maintaining an approximately equimolar synthesis of alpha- and beta-tubulin subunits throughout embryogenesis.
已鉴定出四种不同的微管蛋白，它们在黑腹果蝇胚胎发生过程中表达。两种α-微管蛋白亚基（α1和α2）和一种β-微管蛋白亚基（β1）在整个胚胎发育过程中均有表达。第二种β-微管蛋白亚基（β3）仅在胚胎发育中期短暂表达。在兔网织红细胞翻译系统中体外合成β3-微管蛋白，仅由胚胎在该蛋白表达阶段提取的RNA指导。因此我们得出结论，编码β3-微管蛋白的mRNA仅在β3-微管蛋白合成的短暂时期转录。β3-微管蛋白的表达伴随着胚胎α-微管蛋白合成水平的协同短暂增加，从而在整个胚胎发生过程中维持α-和β-微管蛋白亚基的近似等摩尔合成。
