Stankov B M, Uscheva A A, Kanchev L N
Biochem Int. 1986 Jan;12(1):11-9.
Six different chromatographic procedures were employed to separate the intact labelled ovine follicle stimulating hormone after iodination of a highly purified preparation. The immunoreactivity of the fractions was tested in the conditions of a double-antibody radioimmunoassay. A single point crossreaction was introduced to calculate the interference of luteinizing hormone in the assay. The results obtained after SDS electrophoresis of the hormone were compared with the results of the autoradiography of the fractions subjected to electrophoresis after labelling and purification. Intact hormone with both subunits present was recovered following chromatography on Blue Sepharose C1-6B or high resolution gel filtration on Ultrogel AcA-54.
在对一种高度纯化的制剂进行碘化后，采用六种不同的色谱方法来分离完整的标记绵羊促卵泡激素。在双抗体放射免疫测定条件下测试各馏分的免疫反应性。引入单点交叉反应以计算促黄体生成素在该测定中的干扰。将该激素经十二烷基硫酸钠电泳后得到的结果与标记和纯化后经电泳的各馏分的放射自显影结果进行比较。在蓝色琼脂糖凝胶C1-6B上进行色谱分离或在Ultrogel AcA-54上进行高分辨率凝胶过滤后，回收了同时含有两个亚基的完整激素。
